A vector for expressing foreign genes in the brains and hearts of transgenic mice

David R. Borchelt, Janine Davis, Marek Fischer, Micheal K. Lee, Hilda H. Slunt, Tamara Ratovitsky, Jean Regard, Neal G. Copeland, Nancy A. Jenkins, Sangram S. Sisodia, Donald L. Price

Research output: Contribution to journalArticlepeer-review

310 Scopus citations

Abstract

An expression plasmid (MoPrP.Xho), for use in transgenic mice, was developed from the promoter, 5' intronic, and 3' untranslated sequences of the murine prion protein gene. Analyses of mice harboring the MoPrP.Xho construct with cDNA genes encoding the amyloid precursor protein (APP) and human presenilin I demonstrated that this vector provides relatively high levels of transgene-encoded polypeptides in brains and hearts of transgenic mice. The MoPrP.Xho vector should be very useful in strategies designed to overexpress a variety of wild-type and disease related mutant transgenes in the heart and brain.

Original languageEnglish (US)
Pages (from-to)159-163
Number of pages5
JournalGenetic Analysis - Biomolecular Engineering
Volume13
Issue number6
DOIs
StatePublished - Dec 1996

Keywords

  • Brain
  • Expression vector
  • Transgenic

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

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