TY - JOUR
T1 - Extracellular recordings from patterned neuronal networks using planar microelectrode arrays
AU - James, Conrad D.
AU - Spence, Andrew J H
AU - Dowell-Mesfin, Natalie M.
AU - Hussain, Rifat J.
AU - Smith, Karen L.
AU - Craighead, Harold G.
AU - Isaacson, Michael S.
AU - Shain, William
AU - Turner, James N.
N1 - Funding Information:
Microfabrication was performed at the Cornell Nanofab-rication Facility (a member of the National Nanofabrication Users Network) which is supported by NSF under Grant ECS-9731293, its users, Cornell University and Industrial Affiliates.
Funding Information:
Manuscript received April 19, 2002; revised October 21, 2003. This work supported in part by the (DARPA) and in part by the National Institutes of Health (NIH). The work of C. D. James was supported in part by the National Science Foundation (NSF) under a Graduate Fellowship and in part by the NIH under a Molecular Biophysics Training Grant. The work of A. J. Spence was supported by a W. M. Keck Foundation Fellowship. Asterisk indicates corresponding author. *C. D. James was with Cornell University, Ithaca, NY 14850 USA. He is now with Sandia National Laboratories, Albuquerque, NM 87185 USA (e-mail: [email protected]).
PY - 2004/9
Y1 - 2004/9
N2 - Neuronal cell networks have been reconstructed on planar microelectrode arrays (MEAs) from dissociated hippocampal pyramidal neurons. Microcontact printing (μCP) and a photoresist-liftoff method were used to selectively localize poly-L-lysine (PLL) on the surface of MEAs. Haptotaxis led to the organization of the neurons into networks localized adjacent to microelectrodes. Various grids of PLL with 2-25-μm-wide lines spaced by 50-200 μm with 15-25-μm nodes at intersection points were used to guide cell body attachment and neurite outgrowth. Bursting activity with spike amplitude attenuation was observed, and multichannel recordings detected instances of coincident firing activity. Finally, we present here an extracellular recording from a ∼2 μm bundle of guided neurites.
AB - Neuronal cell networks have been reconstructed on planar microelectrode arrays (MEAs) from dissociated hippocampal pyramidal neurons. Microcontact printing (μCP) and a photoresist-liftoff method were used to selectively localize poly-L-lysine (PLL) on the surface of MEAs. Haptotaxis led to the organization of the neurons into networks localized adjacent to microelectrodes. Various grids of PLL with 2-25-μm-wide lines spaced by 50-200 μm with 15-25-μm nodes at intersection points were used to guide cell body attachment and neurite outgrowth. Bursting activity with spike amplitude attenuation was observed, and multichannel recordings detected instances of coincident firing activity. Finally, we present here an extracellular recording from a ∼2 μm bundle of guided neurites.
KW - Hippocampal pyramidal neuron
KW - Microcontact printing
KW - Microelectrode array
KW - Neuron networks
UR - http://www.scopus.com/inward/record.url?scp=4143059049&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4143059049&partnerID=8YFLogxK
U2 - 10.1109/TBME.2004.827252
DO - 10.1109/TBME.2004.827252
M3 - Article
C2 - 15376512
AN - SCOPUS:4143059049
SN - 0018-9294
VL - 51
SP - 1640
EP - 1648
JO - IEEE Transactions on Biomedical Engineering
JF - IEEE Transactions on Biomedical Engineering
IS - 9
ER -