TY - JOUR
T1 - Functional characterization of the gene encoding RLIM, the corepressor of LIM homeodomain factors
AU - Ostendorff, Heather P.
AU - Bossenz, Michael
AU - Mincheva, Antoaneta
AU - Copeland, Neal G.
AU - Gilbert, Debra J.
AU - Jenkins, Nancy A.
AU - Lichter, Peter
AU - Bach, Ingolf
N1 - Funding Information:
We thank C. Hübner, N. Piwon, M. Wegner, I. Hermans-Borg-meyer, R. Waltereit, B. Bourachot, R. Peirano, M. Yaniv, F. Logeat, A. Israel, M. Crossley, G. Suske, J. Leiden, and V. Poli for providing critical plasmids and reagents and D. B. Householder for excellent technical assistance. H.P.O. is a fellow of the Graduiertenkolleg 255 of the University of Hamburg. This research was supported, in part, by the National Cancer Institute, DHHS, under contract with ABL. I.B. is supported by the Deutsche Forschungsgemeinschaft (BA1842/1-2, 1842/2-1, 1842/3-1, and SFB444).
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2000/10/1
Y1 - 2000/10/1
N2 - RLIM is a RING H2 zinc finger protein that acts as a negative coregulator for LIM homeodomain transcription factors. We have isolated genomic λ clones that cover the entire mouse RLIM-encoding Rnf12 gene. The Rnf12 gene encompasses 20 kb and consists of at least five exons and four introns. Several transcriptional start sites within a 24-bp region were mapped around 300 nt upstream of the translational start site. Rnf12-specific mRNA can be detected in many tissues as revealed by Northern blot analysis. Transient cotransfections reveal that the proximal Rnf12 promoter can be activated in vitro by ubiquitously and more restrictively expressed transcription factors, some of which are known mediators of signal transduction pathways, e.g., mammalian Kruppel-like transcription factors, Sox and ets-related proteins, and RBP-J. We isolated a cDNA encoding human RLIM, which is highly conserved with mouse and chick RLIM. By fluorescence in situ hybridization and interspecific backcross analysis, we have localized the Rnf12 gene to the central regions of mouse and human chromosome X. (C) 2000 Academic Press.
AB - RLIM is a RING H2 zinc finger protein that acts as a negative coregulator for LIM homeodomain transcription factors. We have isolated genomic λ clones that cover the entire mouse RLIM-encoding Rnf12 gene. The Rnf12 gene encompasses 20 kb and consists of at least five exons and four introns. Several transcriptional start sites within a 24-bp region were mapped around 300 nt upstream of the translational start site. Rnf12-specific mRNA can be detected in many tissues as revealed by Northern blot analysis. Transient cotransfections reveal that the proximal Rnf12 promoter can be activated in vitro by ubiquitously and more restrictively expressed transcription factors, some of which are known mediators of signal transduction pathways, e.g., mammalian Kruppel-like transcription factors, Sox and ets-related proteins, and RBP-J. We isolated a cDNA encoding human RLIM, which is highly conserved with mouse and chick RLIM. By fluorescence in situ hybridization and interspecific backcross analysis, we have localized the Rnf12 gene to the central regions of mouse and human chromosome X. (C) 2000 Academic Press.
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U2 - 10.1006/geno.2000.6311
DO - 10.1006/geno.2000.6311
M3 - Article
C2 - 11013082
AN - SCOPUS:0034306780
SN - 0888-7543
VL - 69
SP - 120
EP - 130
JO - Genomics
JF - Genomics
IS - 1
ER -