Intracellular delivery of molecular beacons by PMMA nanoparticles and carbon nanotubes for mRNA sensing

A. Giannetti; S. Tombelli; C. Trono; M. Ballestri; G. Giambastiani; A. Guerrini; G. Sotgiu; G. Tuci; Greta Varchi; F. Baldini

doi:10.1117/12.2007391

Intracellular delivery of molecular beacons by PMMA nanoparticles and carbon nanotubes for mRNA sensing

A. Giannetti, S. Tombelli, C. Trono, M. Ballestri, G. Giambastiani, A. Guerrini, G. Sotgiu, G. Tuci, Greta Varchi, F. Baldini

Houston Methodist

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

4 Scopus citations

Abstract

We describe here the use of carbon nanotubes (CNTs) and polymeric nanoparticles made of a core of polymethylmethacrylate (PMMA) surrounded by a shell bearing cationic groups, as intracellular delivery tools of molecular beacons (MBs), particular fluorescent DNA probes, for the detection and localization of a specific mRNA. Survivin mRNA targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The MB was anchored to the surface of CNTs and PMMA nanoparticles via a commercial sulfhydryl-reactive heterobifunctional crosslinker and the achieved nanomaterials were then characterized in vitro.

Original language	English (US)
Title of host publication	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V
Volume	8596
DOIs	https://doi.org/10.1117/12.2007391
State	Published - May 30 2013
Event	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V - San Francisco, CA, United States Duration: Feb 4 2013 → Feb 6 2013

Other

Other	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V
Country/Territory	United States
City	San Francisco, CA
Period	2/4/13 → 2/6/13

Keywords

Molecular beacon
carbon nanotubes
intracellular delivery
intracellular sensing
polymethylmethacrylate nanoparticles
survivin

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

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10.1117/12.2007391

Cite this

Giannetti, A., Tombelli, S., Trono, C., Ballestri, M., Giambastiani, G., Guerrini, A., Sotgiu, G., Tuci, G., Varchi, G., & Baldini, F. (2013). Intracellular delivery of molecular beacons by PMMA nanoparticles and carbon nanotubes for mRNA sensing. In Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V (Vol. 8596). Article 85960U https://doi.org/10.1117/12.2007391

Giannetti, A, Tombelli, S, Trono, C, Ballestri, M, Giambastiani, G, Guerrini, A, Sotgiu, G, Tuci, G, Varchi, G & Baldini, F 2013, Intracellular delivery of molecular beacons by PMMA nanoparticles and carbon nanotubes for mRNA sensing. in Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V. vol. 8596, 85960U, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V, San Francisco, CA, United States, 2/4/13. https://doi.org/10.1117/12.2007391

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abstract = "We describe here the use of carbon nanotubes (CNTs) and polymeric nanoparticles made of a core of polymethylmethacrylate (PMMA) surrounded by a shell bearing cationic groups, as intracellular delivery tools of molecular beacons (MBs), particular fluorescent DNA probes, for the detection and localization of a specific mRNA. Survivin mRNA targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The MB was anchored to the surface of CNTs and PMMA nanoparticles via a commercial sulfhydryl-reactive heterobifunctional crosslinker and the achieved nanomaterials were then characterized in vitro.",

keywords = "Molecular beacon, carbon nanotubes, intracellular delivery, intracellular sensing, polymethylmethacrylate nanoparticles, survivin",

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AU - Giannetti, A.

AU - Tombelli, S.

AU - Trono, C.

AU - Ballestri, M.

AU - Giambastiani, G.

AU - Guerrini, A.

AU - Sotgiu, G.

AU - Tuci, G.

AU - Varchi, Greta

AU - Baldini, F.

PY - 2013/5/30

Y1 - 2013/5/30

N2 - We describe here the use of carbon nanotubes (CNTs) and polymeric nanoparticles made of a core of polymethylmethacrylate (PMMA) surrounded by a shell bearing cationic groups, as intracellular delivery tools of molecular beacons (MBs), particular fluorescent DNA probes, for the detection and localization of a specific mRNA. Survivin mRNA targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The MB was anchored to the surface of CNTs and PMMA nanoparticles via a commercial sulfhydryl-reactive heterobifunctional crosslinker and the achieved nanomaterials were then characterized in vitro.

AB - We describe here the use of carbon nanotubes (CNTs) and polymeric nanoparticles made of a core of polymethylmethacrylate (PMMA) surrounded by a shell bearing cationic groups, as intracellular delivery tools of molecular beacons (MBs), particular fluorescent DNA probes, for the detection and localization of a specific mRNA. Survivin mRNA targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The MB was anchored to the surface of CNTs and PMMA nanoparticles via a commercial sulfhydryl-reactive heterobifunctional crosslinker and the achieved nanomaterials were then characterized in vitro.

KW - Molecular beacon

KW - carbon nanotubes

KW - intracellular delivery

KW - intracellular sensing

KW - polymethylmethacrylate nanoparticles

KW - survivin

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VL - 8596

BT - Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V

T2 - Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V

Y2 - 4 February 2013 through 6 February 2013

ER -

Intracellular delivery of molecular beacons by PMMA nanoparticles and carbon nanotubes for mRNA sensing

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