Isolation of two genes that affect mitotic chromosome transmission in S. cerevisiae

Douglas Meeks-Wagner; John S. Wood; Barbara Garvik; Leland H. Hartwell

doi:10.1016/0092-8674(86)90484-8

Isolation of two genes that affect mitotic chromosome transmission in S. cerevisiae

Douglas Meeks-Wagner, John S. Wood, Barbara Garvik, Leland H. Hartwell

Houston Methodist

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

Two DNA sequences that reduce mitotic fidelity of chromosome transmission have been identified: MIF1 and MIF2. MIF1 is a unique sequence located on the right arm of chromosome XII that stimulates loss and recombination for both chromosomes V and VII when present in a high copy number plasmid. MIF1 is not essential for cell division but is necessary for the normal fidelity of chromosome transmission. MIF2 is a unique sequence located 15 cM distal to HIS6 on chromosome IX that induces a high frequency of chromosome VII loss and a lower frequency of chromosome V loss when present in high copy number; it has no effect on mitotic recombination. Disruption of the genomic MIF2 locus was lethal and cells lacking this function arrested division with a terminal phenotype characteristic of a block in DNA replication or nuclear division.

Original language	English (US)
Pages (from-to)	53-63
Number of pages	11
Journal	Cell
Volume	44
Issue number	1
DOIs	https://doi.org/10.1016/0092-8674(86)90484-8
State	Published - Jan 17 1986

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(86)90484-8

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title = "Isolation of two genes that affect mitotic chromosome transmission in S. cerevisiae",

abstract = "Two DNA sequences that reduce mitotic fidelity of chromosome transmission have been identified: MIF1 and MIF2. MIF1 is a unique sequence located on the right arm of chromosome XII that stimulates loss and recombination for both chromosomes V and VII when present in a high copy number plasmid. MIF1 is not essential for cell division but is necessary for the normal fidelity of chromosome transmission. MIF2 is a unique sequence located 15 cM distal to HIS6 on chromosome IX that induces a high frequency of chromosome VII loss and a lower frequency of chromosome V loss when present in high copy number; it has no effect on mitotic recombination. Disruption of the genomic MIF2 locus was lethal and cells lacking this function arrested division with a terminal phenotype characteristic of a block in DNA replication or nuclear division.",

author = "Douglas Meeks-Wagner and Wood, {John S.} and Barbara Garvik and Hartwell, {Leland H.}",

note = "Funding Information: We thank Sherie Hartwell for technical assistance. We are grateful to Megan Brown, Douglas Koshland, Dan Burke, Walt Fangman, Breck Byers, and Bonita Brewer for helpful discussions of our work, and for their comments on this manuscript. This research was supported by the National Science Foundation under grant PCM 8215113 and the Funding Information: National Institutes of Health research grant GM17709 from the division of General Medical Sciences.",

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T1 - Isolation of two genes that affect mitotic chromosome transmission in S. cerevisiae

AU - Meeks-Wagner, Douglas

AU - Wood, John S.

AU - Garvik, Barbara

AU - Hartwell, Leland H.

N1 - Funding Information: We thank Sherie Hartwell for technical assistance. We are grateful to Megan Brown, Douglas Koshland, Dan Burke, Walt Fangman, Breck Byers, and Bonita Brewer for helpful discussions of our work, and for their comments on this manuscript. This research was supported by the National Science Foundation under grant PCM 8215113 and the Funding Information: National Institutes of Health research grant GM17709 from the division of General Medical Sciences.

PY - 1986/1/17

Y1 - 1986/1/17

N2 - Two DNA sequences that reduce mitotic fidelity of chromosome transmission have been identified: MIF1 and MIF2. MIF1 is a unique sequence located on the right arm of chromosome XII that stimulates loss and recombination for both chromosomes V and VII when present in a high copy number plasmid. MIF1 is not essential for cell division but is necessary for the normal fidelity of chromosome transmission. MIF2 is a unique sequence located 15 cM distal to HIS6 on chromosome IX that induces a high frequency of chromosome VII loss and a lower frequency of chromosome V loss when present in high copy number; it has no effect on mitotic recombination. Disruption of the genomic MIF2 locus was lethal and cells lacking this function arrested division with a terminal phenotype characteristic of a block in DNA replication or nuclear division.

AB - Two DNA sequences that reduce mitotic fidelity of chromosome transmission have been identified: MIF1 and MIF2. MIF1 is a unique sequence located on the right arm of chromosome XII that stimulates loss and recombination for both chromosomes V and VII when present in a high copy number plasmid. MIF1 is not essential for cell division but is necessary for the normal fidelity of chromosome transmission. MIF2 is a unique sequence located 15 cM distal to HIS6 on chromosome IX that induces a high frequency of chromosome VII loss and a lower frequency of chromosome V loss when present in high copy number; it has no effect on mitotic recombination. Disruption of the genomic MIF2 locus was lethal and cells lacking this function arrested division with a terminal phenotype characteristic of a block in DNA replication or nuclear division.

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Isolation of two genes that affect mitotic chromosome transmission in S. cerevisiae

Abstract

ASJC Scopus subject areas

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