Label-free optical detection of anthrax-causing spores

Ghanashyam Acharya; Derek D. Doorneweerd; Chun Li Chang; Walter A. Henne; Philip S. Low; Cagri A. Savran

doi:10.1021/ja0656649

Label-free optical detection of anthrax-causing spores

Ghanashyam Acharya, Derek D. Doorneweerd, Chun Li Chang, Walter A. Henne, Philip S. Low, Cagri A. Savran

Houston Methodist

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

An optical biosensor integrated with short peptide ligands for detection of Bacillus anthracis spores is described. A short peptide that can bind specifically to B. anthracis spores was synthesized and used as the ligand. With this approach, a minimum of 34 spores of B. anthracis, specifically captured on the sensor, were detected by measuring the laser light transmission intensity and verified by laser scanning confocal fluorescence microscopy. The differential nature of measurement reduces the incidence of false positives. The detection process, from capturing B. anthracis spores on the sensor surface to laser transmission intensity measurements, required a short time of 35 min. This biosensor, with its simple instrumentation, ease of use, and short detection time, can enable the development of a portable device for rapid, label-free, specific, and quantitative detection of anthrax-causing spores.

Original language	English (US)
Pages (from-to)	732-733
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	129
Issue number	4
DOIs	https://doi.org/10.1021/ja0656649
State	Published - Jan 31 2007

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja0656649

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title = "Label-free optical detection of anthrax-causing spores",

abstract = "An optical biosensor integrated with short peptide ligands for detection of Bacillus anthracis spores is described. A short peptide that can bind specifically to B. anthracis spores was synthesized and used as the ligand. With this approach, a minimum of 34 spores of B. anthracis, specifically captured on the sensor, were detected by measuring the laser light transmission intensity and verified by laser scanning confocal fluorescence microscopy. The differential nature of measurement reduces the incidence of false positives. The detection process, from capturing B. anthracis spores on the sensor surface to laser transmission intensity measurements, required a short time of 35 min. This biosensor, with its simple instrumentation, ease of use, and short detection time, can enable the development of a portable device for rapid, label-free, specific, and quantitative detection of anthrax-causing spores.",

author = "Ghanashyam Acharya and Doorneweerd, {Derek D.} and Chang, {Chun Li} and Henne, {Walter A.} and Low, {Philip S.} and Savran, {Cagri A.}",

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AU - Acharya, Ghanashyam

AU - Doorneweerd, Derek D.

AU - Chang, Chun Li

AU - Henne, Walter A.

AU - Low, Philip S.

AU - Savran, Cagri A.

PY - 2007/1/31

Y1 - 2007/1/31

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AB - An optical biosensor integrated with short peptide ligands for detection of Bacillus anthracis spores is described. A short peptide that can bind specifically to B. anthracis spores was synthesized and used as the ligand. With this approach, a minimum of 34 spores of B. anthracis, specifically captured on the sensor, were detected by measuring the laser light transmission intensity and verified by laser scanning confocal fluorescence microscopy. The differential nature of measurement reduces the incidence of false positives. The detection process, from capturing B. anthracis spores on the sensor surface to laser transmission intensity measurements, required a short time of 35 min. This biosensor, with its simple instrumentation, ease of use, and short detection time, can enable the development of a portable device for rapid, label-free, specific, and quantitative detection of anthrax-causing spores.

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Label-free optical detection of anthrax-causing spores

Abstract

ASJC Scopus subject areas

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