Molecular cloning and biological characterization of a novel murine lymphoid growth factor

John E. Sims; Douglas E. Williams; Philip J. Morrissey; Kirsten Garka; Diane Foxworthe; Virginia Price; Sherree L. Friend; Andrew Farr; Mary A. Bedell; Nancy A. Jenkins; Neal G. Copeland; Kenneth Grabstein; Raymond J. Paxton

doi:10.1084/jem.192.5.671

Molecular cloning and biological characterization of a novel murine lymphoid growth factor

John E. Sims, Douglas E. Williams, Philip J. Morrissey, Kirsten Garka, Diane Foxworthe, Virginia Price, Sherree L. Friend, Andrew Farr, Mary A. Bedell, Nancy A. Jenkins, Neal G. Copeland, Kenneth Grabstein, Raymond J. Paxton

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197 Scopus citations

Abstract

Using a bioassay consisting of the proliferation of a murine B cell line, a cDNA of a gene whose product supports the growth of that cell line was isolated from a thymic stromal cell line. This factor, termed thymic stromal lymphopoietin (TSLP), is a protein of 140 amino acids. The gene encoding TSLP was mapped to murine chromosome 18. Purified recombinant TSLP supported the growth of pre-B cell colonies in vitro, but had no myelopoietic activity. TSLP had comitogenic activity for fetal thymocytes, but was not as potent as interleukin 7 in lobe submersion cultures. Injection of TSLP into neonatal mice induced the expansion of B220⁺BP-1⁺ pre-B cells.

Original language	English (US)
Pages (from-to)	671-680
Number of pages	10
Journal	Journal of Experimental Medicine
Volume	192
Issue number	5
DOIs	https://doi.org/10.1084/jem.192.5.671
State	Published - Sep 4 2000

Keywords

B lymphocyte
Cytokine
DNA sequence
Growth factor
Lymphocyte differentiation

ASJC Scopus subject areas

Immunology

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Sims, J. E., Williams, D. E., Morrissey, P. J., Garka, K., Foxworthe, D., Price, V., Friend, S. L., Farr, A., Bedell, M. A., Jenkins, N. A., Copeland, N. G., Grabstein, K., & Paxton, R. J. (2000). Molecular cloning and biological characterization of a novel murine lymphoid growth factor. Journal of Experimental Medicine, 192(5), 671-680. https://doi.org/10.1084/jem.192.5.671

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abstract = "Using a bioassay consisting of the proliferation of a murine B cell line, a cDNA of a gene whose product supports the growth of that cell line was isolated from a thymic stromal cell line. This factor, termed thymic stromal lymphopoietin (TSLP), is a protein of 140 amino acids. The gene encoding TSLP was mapped to murine chromosome 18. Purified recombinant TSLP supported the growth of pre-B cell colonies in vitro, but had no myelopoietic activity. TSLP had comitogenic activity for fetal thymocytes, but was not as potent as interleukin 7 in lobe submersion cultures. Injection of TSLP into neonatal mice induced the expansion of B220+BP-1+ pre-B cells.",

keywords = "B lymphocyte, Cytokine, DNA sequence, Growth factor, Lymphocyte differentiation",

author = "Sims, {John E.} and Williams, {Douglas E.} and Morrissey, {Philip J.} and Kirsten Garka and Diane Foxworthe and Virginia Price and Friend, {Sherree L.} and Andrew Farr and Bedell, {Mary A.} and Jenkins, {Nancy A.} and Copeland, {Neal G.} and Kenneth Grabstein and Paxton, {Raymond J.}",

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AU - Sims, John E.

AU - Williams, Douglas E.

AU - Morrissey, Philip J.

AU - Garka, Kirsten

AU - Foxworthe, Diane

AU - Price, Virginia

AU - Friend, Sherree L.

AU - Farr, Andrew

AU - Bedell, Mary A.

AU - Jenkins, Nancy A.

AU - Copeland, Neal G.

AU - Grabstein, Kenneth

AU - Paxton, Raymond J.

PY - 2000/9/4

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AB - Using a bioassay consisting of the proliferation of a murine B cell line, a cDNA of a gene whose product supports the growth of that cell line was isolated from a thymic stromal cell line. This factor, termed thymic stromal lymphopoietin (TSLP), is a protein of 140 amino acids. The gene encoding TSLP was mapped to murine chromosome 18. Purified recombinant TSLP supported the growth of pre-B cell colonies in vitro, but had no myelopoietic activity. TSLP had comitogenic activity for fetal thymocytes, but was not as potent as interleukin 7 in lobe submersion cultures. Injection of TSLP into neonatal mice induced the expansion of B220+BP-1+ pre-B cells.

KW - B lymphocyte

KW - Cytokine

KW - DNA sequence

KW - Growth factor

KW - Lymphocyte differentiation

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EP - 680

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

IS - 5

ER -

Molecular cloning and biological characterization of a novel murine lymphoid growth factor

Abstract

Keywords

ASJC Scopus subject areas

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