Non-cytolytic antigen clearance in DNA-vaccinated mice with electroporation

Jin Liang Peng; Yong Gang Zhao; Jun Hua Mai; Wen Ka Pang; Wei Guo; Guang Ming Chen; Guo Yu Mo; Guirong Rao; Yu Hong Xu

doi:10.1111/j.1745-7254.2007.00591.x

Non-cytolytic antigen clearance in DNA-vaccinated mice with electroporation

Jin Liang Peng, Yong Gang Zhao, Jun Hua Mai, Wen Ka Pang, Wei Guo, Guang Ming Chen, Guo Yu Mo, Guirong Rao, Yu Hong Xu

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Aim: To explore the potential of electroporation (EP)-mediated hepatitis B virus (HBV) DNA vaccination for the treatment of chronic HBV infection. Methods: BALB/c mice were vaccinated with HBV DNA vaccine encoding for the HBV preS ₂-S antigen, combined with or without EP. HBV surface antigen expression plasmid was administered into mice liver via a hydrodynamic injection to mimic HBV infection. The clearance of antigen in the serum and liver was detected by ELISA assay and immunohistochemical staining. The histopathology of the liver tissues was examined by HE staining and serum alanine aminotransferase assay. Results: The immunogenicity of HBV DNA vaccine encoding for the HBV preS₂-S antigen can be improved by EP-mediated vaccine delivery. The elicited immune responses can indeed reduce the expression of HBV surface antigen (HBsAg) in hepatocytes of the mouse model that was transfected to express HBsAg using the hydrodynamic injection method. The antigen clearance process did not cause significant toxicity to liver tissue, suggesting a non-cytolytic mechanism. Conclusion: The EP-aided DNA vaccination may have potential in mediating viral clearance in chronic hepatitis B patients.

Original language	English (US)
Pages (from-to)	1024-1030
Number of pages	7
Journal	Acta Pharmacologica Sinica
Volume	28
Issue number	7
DOIs	https://doi.org/10.1111/j.1745-7254.2007.00591.x
State	Published - Jul 2007

Keywords

Antigen clearance
DNA vaccine
Electroporation
Hepatitis B virus

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)

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10.1111/j.1745-7254.2007.00591.x

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title = "Non-cytolytic antigen clearance in DNA-vaccinated mice with electroporation",

abstract = "Aim: To explore the potential of electroporation (EP)-mediated hepatitis B virus (HBV) DNA vaccination for the treatment of chronic HBV infection. Methods: BALB/c mice were vaccinated with HBV DNA vaccine encoding for the HBV preS 2-S antigen, combined with or without EP. HBV surface antigen expression plasmid was administered into mice liver via a hydrodynamic injection to mimic HBV infection. The clearance of antigen in the serum and liver was detected by ELISA assay and immunohistochemical staining. The histopathology of the liver tissues was examined by HE staining and serum alanine aminotransferase assay. Results: The immunogenicity of HBV DNA vaccine encoding for the HBV preS2-S antigen can be improved by EP-mediated vaccine delivery. The elicited immune responses can indeed reduce the expression of HBV surface antigen (HBsAg) in hepatocytes of the mouse model that was transfected to express HBsAg using the hydrodynamic injection method. The antigen clearance process did not cause significant toxicity to liver tissue, suggesting a non-cytolytic mechanism. Conclusion: The EP-aided DNA vaccination may have potential in mediating viral clearance in chronic hepatitis B patients.",

keywords = "Antigen clearance, DNA vaccine, Electroporation, Hepatitis B virus",

author = "Peng, {Jin Liang} and Zhao, {Yong Gang} and Mai, {Jun Hua} and Pang, {Wen Ka} and Wei Guo and Chen, {Guang Ming} and Mo, {Guo Yu} and Guirong Rao and Xu, {Yu Hong}",

year = "2007",

month = jul,

doi = "10.1111/j.1745-7254.2007.00591.x",

language = "English (US)",

volume = "28",

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T1 - Non-cytolytic antigen clearance in DNA-vaccinated mice with electroporation

AU - Peng, Jin Liang

AU - Zhao, Yong Gang

AU - Mai, Jun Hua

AU - Pang, Wen Ka

AU - Guo, Wei

AU - Chen, Guang Ming

AU - Mo, Guo Yu

AU - Rao, Guirong

AU - Xu, Yu Hong

PY - 2007/7

Y1 - 2007/7

N2 - Aim: To explore the potential of electroporation (EP)-mediated hepatitis B virus (HBV) DNA vaccination for the treatment of chronic HBV infection. Methods: BALB/c mice were vaccinated with HBV DNA vaccine encoding for the HBV preS 2-S antigen, combined with or without EP. HBV surface antigen expression plasmid was administered into mice liver via a hydrodynamic injection to mimic HBV infection. The clearance of antigen in the serum and liver was detected by ELISA assay and immunohistochemical staining. The histopathology of the liver tissues was examined by HE staining and serum alanine aminotransferase assay. Results: The immunogenicity of HBV DNA vaccine encoding for the HBV preS2-S antigen can be improved by EP-mediated vaccine delivery. The elicited immune responses can indeed reduce the expression of HBV surface antigen (HBsAg) in hepatocytes of the mouse model that was transfected to express HBsAg using the hydrodynamic injection method. The antigen clearance process did not cause significant toxicity to liver tissue, suggesting a non-cytolytic mechanism. Conclusion: The EP-aided DNA vaccination may have potential in mediating viral clearance in chronic hepatitis B patients.

AB - Aim: To explore the potential of electroporation (EP)-mediated hepatitis B virus (HBV) DNA vaccination for the treatment of chronic HBV infection. Methods: BALB/c mice were vaccinated with HBV DNA vaccine encoding for the HBV preS 2-S antigen, combined with or without EP. HBV surface antigen expression plasmid was administered into mice liver via a hydrodynamic injection to mimic HBV infection. The clearance of antigen in the serum and liver was detected by ELISA assay and immunohistochemical staining. The histopathology of the liver tissues was examined by HE staining and serum alanine aminotransferase assay. Results: The immunogenicity of HBV DNA vaccine encoding for the HBV preS2-S antigen can be improved by EP-mediated vaccine delivery. The elicited immune responses can indeed reduce the expression of HBV surface antigen (HBsAg) in hepatocytes of the mouse model that was transfected to express HBsAg using the hydrodynamic injection method. The antigen clearance process did not cause significant toxicity to liver tissue, suggesting a non-cytolytic mechanism. Conclusion: The EP-aided DNA vaccination may have potential in mediating viral clearance in chronic hepatitis B patients.

KW - Antigen clearance

KW - DNA vaccine

KW - Electroporation

KW - Hepatitis B virus

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Non-cytolytic antigen clearance in DNA-vaccinated mice with electroporation

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