Normal forebrain development may require continual Wnt antagonism until mid-somitogenesis in zebrafish

Jun Dae Kim; Hang Suk Chun; Seok Hyung Kim; Hyung Seok Kim; Young Seop Kim; Myoung Jin Kim; Jimann Shin; Myungchull Rhee; Sang Yeob Yeo; Tae Lin Huh

doi:10.1016/j.bbrc.2009.02.135

Normal forebrain development may require continual Wnt antagonism until mid-somitogenesis in zebrafish

Jun Dae Kim, Hang Suk Chun, Seok Hyung Kim, Hyung Seok Kim, Young Seop Kim, Myoung Jin Kim, Jimann Shin, Myungchull Rhee, Sang Yeob Yeo, Tae Lin Huh

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

During normal forebrain development in vertebrates, rostral neural tissue must be protected from Wnt signals via the actions of locally expressed Wnt antagonistic factors. In zebrafish zygotic oep (Zoep) mutants, forebrain structure is severely disrupted with reduced expression of the Wnt antagonists secreted frizzled related protein1 and dickkopf1. To analyze the temporal effects of Wnt antagonism on forebrain development, we generated transgenic zebrafish that overexpressed the dominant negative form of frizzled8a (DNfz8a) in wild-type and Zoep mutants under the control of a heat-inducible promoter. This model allowed for assessment of the dynamics of Wnt antagonistic signaling during forebrain development. Our results demonstrated that overexpression of DNfz8a in Zoep embryos between 7 and 16 hpf increased putative forebrain region demarcated by anf and distal-less2 expressions. These results suggest that normal forebrain development requires continual Wnt antagonism from the early gastrula to the mid-somitogenesis stage.

Original language	English (US)
Pages (from-to)	717-721
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	381
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2009.02.135
State	Published - Apr 17 2009

Keywords

Forebrain development
Transgenic zebrafish
Wnt antagonist
Zygotic one-eyed pinhead (oep) mutant
frizzled8a

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2009.02.135

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title = "Normal forebrain development may require continual Wnt antagonism until mid-somitogenesis in zebrafish",

abstract = "During normal forebrain development in vertebrates, rostral neural tissue must be protected from Wnt signals via the actions of locally expressed Wnt antagonistic factors. In zebrafish zygotic oep (Zoep) mutants, forebrain structure is severely disrupted with reduced expression of the Wnt antagonists secreted frizzled related protein1 and dickkopf1. To analyze the temporal effects of Wnt antagonism on forebrain development, we generated transgenic zebrafish that overexpressed the dominant negative form of frizzled8a (DNfz8a) in wild-type and Zoep mutants under the control of a heat-inducible promoter. This model allowed for assessment of the dynamics of Wnt antagonistic signaling during forebrain development. Our results demonstrated that overexpression of DNfz8a in Zoep embryos between 7 and 16 hpf increased putative forebrain region demarcated by anf and distal-less2 expressions. These results suggest that normal forebrain development requires continual Wnt antagonism from the early gastrula to the mid-somitogenesis stage.",

keywords = "Forebrain development, Transgenic zebrafish, Wnt antagonist, Zygotic one-eyed pinhead (oep) mutant, frizzled8a",

author = "Kim, {Jun Dae} and Chun, {Hang Suk} and Kim, {Seok Hyung} and Kim, {Hyung Seok} and Kim, {Young Seop} and Kim, {Myoung Jin} and Jimann Shin and Myungchull Rhee and Yeo, {Sang Yeob} and Huh, {Tae Lin}",

note = "Funding Information: This research was supported by the Kyungpook National University Research Fund, 2005. We thank the Korea Zebrafish Organogenesis Mutant Bank for technical support and for maintaining the zebrafish lines. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

year = "2009",

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doi = "10.1016/j.bbrc.2009.02.135",

language = "English (US)",

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T1 - Normal forebrain development may require continual Wnt antagonism until mid-somitogenesis in zebrafish

AU - Kim, Jun Dae

AU - Chun, Hang Suk

AU - Kim, Seok Hyung

AU - Kim, Hyung Seok

AU - Kim, Young Seop

AU - Kim, Myoung Jin

AU - Shin, Jimann

AU - Rhee, Myungchull

AU - Yeo, Sang Yeob

AU - Huh, Tae Lin

N1 - Funding Information: This research was supported by the Kyungpook National University Research Fund, 2005. We thank the Korea Zebrafish Organogenesis Mutant Bank for technical support and for maintaining the zebrafish lines. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2009/4/17

Y1 - 2009/4/17

N2 - During normal forebrain development in vertebrates, rostral neural tissue must be protected from Wnt signals via the actions of locally expressed Wnt antagonistic factors. In zebrafish zygotic oep (Zoep) mutants, forebrain structure is severely disrupted with reduced expression of the Wnt antagonists secreted frizzled related protein1 and dickkopf1. To analyze the temporal effects of Wnt antagonism on forebrain development, we generated transgenic zebrafish that overexpressed the dominant negative form of frizzled8a (DNfz8a) in wild-type and Zoep mutants under the control of a heat-inducible promoter. This model allowed for assessment of the dynamics of Wnt antagonistic signaling during forebrain development. Our results demonstrated that overexpression of DNfz8a in Zoep embryos between 7 and 16 hpf increased putative forebrain region demarcated by anf and distal-less2 expressions. These results suggest that normal forebrain development requires continual Wnt antagonism from the early gastrula to the mid-somitogenesis stage.

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Normal forebrain development may require continual Wnt antagonism until mid-somitogenesis in zebrafish

Abstract

Keywords

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