Optogenetic Control of Voltage-Gated Calcium Channels

Guolin Ma; Jindou Liu; Yuepeng Ke; Xin Liu; Minyong Li; Fen Wang; Gang Han; Yun Huang; Youjun Wang; Yubin Zhou

doi:10.1002/anie.201713080

Optogenetic Control of Voltage-Gated Calcium Channels

Guolin Ma, Jindou Liu, Yuepeng Ke, Xin Liu, Minyong Li, Fen Wang, Gang Han, Yun Huang, Youjun Wang, Yubin Zhou

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Voltage-gated Ca²⁺ (Ca_V) channels mediate Ca²⁺ entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded Ca_V channel modulators, to enable photo-tunable modulation of Ca_V channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for Ca_V channels could find broad applications in interrogating a wide range of Ca_V-mediated physiological processes.

Original language	English (US)
Pages (from-to)	7019-7022
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	57
Issue number	24
DOIs	https://doi.org/10.1002/anie.201713080
State	Published - Jun 11 2018

Keywords

calcium signaling
ion channels
optogenetics
protein engineering
synthetic biology

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.201713080

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title = "Optogenetic Control of Voltage-Gated Calcium Channels",

abstract = "Voltage-gated Ca2+ (CaV) channels mediate Ca2+ entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded CaV channel modulators, to enable photo-tunable modulation of CaV channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for CaV channels could find broad applications in interrogating a wide range of CaV-mediated physiological processes.",

keywords = "calcium signaling, ion channels, optogenetics, protein engineering, synthetic biology",

author = "Guolin Ma and Jindou Liu and Yuepeng Ke and Xin Liu and Minyong Li and Fen Wang and Gang Han and Yun Huang and Youjun Wang and Yubin Zhou",

note = "Publisher Copyright: {\textcopyright} 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.",

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T1 - Optogenetic Control of Voltage-Gated Calcium Channels

AU - Ma, Guolin

AU - Liu, Jindou

AU - Ke, Yuepeng

AU - Liu, Xin

AU - Li, Minyong

AU - Wang, Fen

AU - Han, Gang

AU - Huang, Yun

AU - Wang, Youjun

AU - Zhou, Yubin

PY - 2018/6/11

Y1 - 2018/6/11

N2 - Voltage-gated Ca2+ (CaV) channels mediate Ca2+ entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded CaV channel modulators, to enable photo-tunable modulation of CaV channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for CaV channels could find broad applications in interrogating a wide range of CaV-mediated physiological processes.

AB - Voltage-gated Ca2+ (CaV) channels mediate Ca2+ entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded CaV channel modulators, to enable photo-tunable modulation of CaV channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for CaV channels could find broad applications in interrogating a wide range of CaV-mediated physiological processes.

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KW - ion channels

KW - optogenetics

KW - protein engineering

KW - synthetic biology

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JF - Angewandte Chemie - International Edition

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Optogenetic Control of Voltage-Gated Calcium Channels

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Keywords

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