Organization of the mouse microfibril-associated glycoprotein-2 (MAGP- 2) gene

Cheryl Frankfater; Erika Maus; Krisztina Gaal; Fernando Segade; Neal G. Copeland; Debra J. Gilbert; Nancy A. Jenkins; J. Michael Shipley

doi:10.1007/s003350010036

Organization of the mouse microfibril-associated glycoprotein-2 (MAGP- 2) gene

Cheryl Frankfater, Erika Maus, Krisztina Gaal, Fernando Segade, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, J. Michael Shipley

Research output: Contribution to journal › Article › peer-review

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Abstract

A 1.4-kb EST clone encoding mouse microfibril-associated glycoprotein-2 (MAGP-2), identified by its similarity with the reported human cDNA, was used to screen a mouse 129 genomic bacterial artificial chromosome (BAC) library. The mouse gene contains 10 exons spanning 16 kb, located on the distal region of Chromosome (Chr) 6. The exons range in size from 24 to 963 bp, with the ATG located in exon 2. The tenth and largest exon contains 817 bp of 3' untranslated sequence, including a B2 repetitive element. Northern analysis demonstrates abundant expression of MAGP-2 mRNA in skeletal muscle, lung, and heart. Sequence analysis of additional cDNA clones suggests that the two mRNA forms of MAGP-2 in the mouse arise from alternative polyadenylation site usage. The promoter does not contain an obvious TATA box, and the sequence surrounding the start site does not conform to the consensus for an initiator promoter element. Additionally, the mouse promoter contains 22 copies of a CT dinucleotide repeat sequence located ~155 bp 5' to exon 1.

Original language	English (US)
Pages (from-to)	191-195
Number of pages	5
Journal	Mammalian Genome
Volume	11
Issue number	3
DOIs	https://doi.org/10.1007/s003350010036
State	Published - Mar 15 2000

ASJC Scopus subject areas

Genetics

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title = "Organization of the mouse microfibril-associated glycoprotein-2 (MAGP- 2) gene",

abstract = "A 1.4-kb EST clone encoding mouse microfibril-associated glycoprotein-2 (MAGP-2), identified by its similarity with the reported human cDNA, was used to screen a mouse 129 genomic bacterial artificial chromosome (BAC) library. The mouse gene contains 10 exons spanning 16 kb, located on the distal region of Chromosome (Chr) 6. The exons range in size from 24 to 963 bp, with the ATG located in exon 2. The tenth and largest exon contains 817 bp of 3' untranslated sequence, including a B2 repetitive element. Northern analysis demonstrates abundant expression of MAGP-2 mRNA in skeletal muscle, lung, and heart. Sequence analysis of additional cDNA clones suggests that the two mRNA forms of MAGP-2 in the mouse arise from alternative polyadenylation site usage. The promoter does not contain an obvious TATA box, and the sequence surrounding the start site does not conform to the consensus for an initiator promoter element. Additionally, the mouse promoter contains 22 copies of a CT dinucleotide repeat sequence located ~155 bp 5' to exon 1.",

author = "Cheryl Frankfater and Erika Maus and Krisztina Gaal and Fernando Segade and Copeland, {Neal G.} and Gilbert, {Debra J.} and Jenkins, {Nancy A.} and Shipley, {J. Michael}",

year = "2000",

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AU - Frankfater, Cheryl

AU - Maus, Erika

AU - Gaal, Krisztina

AU - Segade, Fernando

AU - Copeland, Neal G.

AU - Gilbert, Debra J.

AU - Jenkins, Nancy A.

AU - Shipley, J. Michael

PY - 2000/3/15

Y1 - 2000/3/15

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AB - A 1.4-kb EST clone encoding mouse microfibril-associated glycoprotein-2 (MAGP-2), identified by its similarity with the reported human cDNA, was used to screen a mouse 129 genomic bacterial artificial chromosome (BAC) library. The mouse gene contains 10 exons spanning 16 kb, located on the distal region of Chromosome (Chr) 6. The exons range in size from 24 to 963 bp, with the ATG located in exon 2. The tenth and largest exon contains 817 bp of 3' untranslated sequence, including a B2 repetitive element. Northern analysis demonstrates abundant expression of MAGP-2 mRNA in skeletal muscle, lung, and heart. Sequence analysis of additional cDNA clones suggests that the two mRNA forms of MAGP-2 in the mouse arise from alternative polyadenylation site usage. The promoter does not contain an obvious TATA box, and the sequence surrounding the start site does not conform to the consensus for an initiator promoter element. Additionally, the mouse promoter contains 22 copies of a CT dinucleotide repeat sequence located ~155 bp 5' to exon 1.

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Organization of the mouse microfibril-associated glycoprotein-2 (MAGP- 2) gene

Abstract

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