Overexpression of DHX32 contributes to the growth and metastasis of colorectal cancer

Huayue Lin; Wenjuan Liu; Zanxi Fang; Xianming Liang; Juan Li; Yongying Bai; Lingqing Lin; Hanyu You; Yihua Pei; Fen Wang; Zhong Ying Zhang

doi:10.1038/srep09247

Overexpression of DHX32 contributes to the growth and metastasis of colorectal cancer

Huayue Lin, Wenjuan Liu, Zanxi Fang, Xianming Liang, Juan Li, Yongying Bai, Lingqing Lin, Hanyu You, Yihua Pei, Fen Wang, Zhong Ying Zhang

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21 Scopus citations

Abstract

Our previous work demonstrates that DHX32 is upregulated in colorectal cancer (CRC) compared to its adjacent normal tissues. However, how overexpressed DHX32 contributes to CRC remains largely unknown. In this study, we reported that DHX32 was overexpressed in human colon cancer cells. Overexpressed DHX32 promoted SW480 cancer cells proliferation, migration, and invasion, as well as decreased the susceptibility to chemotherapy agent 5-Fluorouracil. Furthermore, PCR array analyses revealed that depleting DHX32 in SW480 colon cancer cells suppressed expression of WISP1, MMP7 and VEGFA in the Wnt pathway, and anti-apoptotic gene BCL2 and CA9, however, elevated expression of pro-apoptotic gene ACSL5. The findings suggested that overexpressed DHX32 played an important role in CRC progression and metastasis and that DHX32 has the potential to serve as a biomarker and a novel therapeutic target for CRC.

Original language	English (US)
Article number	9247
Journal	Scientific Reports
Volume	5
DOIs	https://doi.org/10.1038/srep09247
State	Published - Mar 18 2015

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title = "Overexpression of DHX32 contributes to the growth and metastasis of colorectal cancer",

abstract = "Our previous work demonstrates that DHX32 is upregulated in colorectal cancer (CRC) compared to its adjacent normal tissues. However, how overexpressed DHX32 contributes to CRC remains largely unknown. In this study, we reported that DHX32 was overexpressed in human colon cancer cells. Overexpressed DHX32 promoted SW480 cancer cells proliferation, migration, and invasion, as well as decreased the susceptibility to chemotherapy agent 5-Fluorouracil. Furthermore, PCR array analyses revealed that depleting DHX32 in SW480 colon cancer cells suppressed expression of WISP1, MMP7 and VEGFA in the Wnt pathway, and anti-apoptotic gene BCL2 and CA9, however, elevated expression of pro-apoptotic gene ACSL5. The findings suggested that overexpressed DHX32 played an important role in CRC progression and metastasis and that DHX32 has the potential to serve as a biomarker and a novel therapeutic target for CRC.",

author = "Huayue Lin and Wenjuan Liu and Zanxi Fang and Xianming Liang and Juan Li and Yongying Bai and Lingqing Lin and Hanyu You and Yihua Pei and Fen Wang and Zhang, {Zhong Ying}",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (Grant No 813111200, 81401971) and Postdoctoral Science Foundation of China (Grant No 2014M561865).",

year = "2015",

month = mar,

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doi = "10.1038/srep09247",

language = "English (US)",

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T1 - Overexpression of DHX32 contributes to the growth and metastasis of colorectal cancer

AU - Lin, Huayue

AU - Liu, Wenjuan

AU - Fang, Zanxi

AU - Liang, Xianming

AU - Li, Juan

AU - Bai, Yongying

AU - Lin, Lingqing

AU - You, Hanyu

AU - Pei, Yihua

AU - Wang, Fen

AU - Zhang, Zhong Ying

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (Grant No 813111200, 81401971) and Postdoctoral Science Foundation of China (Grant No 2014M561865).

PY - 2015/3/18

Y1 - 2015/3/18

N2 - Our previous work demonstrates that DHX32 is upregulated in colorectal cancer (CRC) compared to its adjacent normal tissues. However, how overexpressed DHX32 contributes to CRC remains largely unknown. In this study, we reported that DHX32 was overexpressed in human colon cancer cells. Overexpressed DHX32 promoted SW480 cancer cells proliferation, migration, and invasion, as well as decreased the susceptibility to chemotherapy agent 5-Fluorouracil. Furthermore, PCR array analyses revealed that depleting DHX32 in SW480 colon cancer cells suppressed expression of WISP1, MMP7 and VEGFA in the Wnt pathway, and anti-apoptotic gene BCL2 and CA9, however, elevated expression of pro-apoptotic gene ACSL5. The findings suggested that overexpressed DHX32 played an important role in CRC progression and metastasis and that DHX32 has the potential to serve as a biomarker and a novel therapeutic target for CRC.

AB - Our previous work demonstrates that DHX32 is upregulated in colorectal cancer (CRC) compared to its adjacent normal tissues. However, how overexpressed DHX32 contributes to CRC remains largely unknown. In this study, we reported that DHX32 was overexpressed in human colon cancer cells. Overexpressed DHX32 promoted SW480 cancer cells proliferation, migration, and invasion, as well as decreased the susceptibility to chemotherapy agent 5-Fluorouracil. Furthermore, PCR array analyses revealed that depleting DHX32 in SW480 colon cancer cells suppressed expression of WISP1, MMP7 and VEGFA in the Wnt pathway, and anti-apoptotic gene BCL2 and CA9, however, elevated expression of pro-apoptotic gene ACSL5. The findings suggested that overexpressed DHX32 played an important role in CRC progression and metastasis and that DHX32 has the potential to serve as a biomarker and a novel therapeutic target for CRC.

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Overexpression of DHX32 contributes to the growth and metastasis of colorectal cancer

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