@article{514b1c21b0004e68943ee656730c0cf8,
title = "Platforms to test the temporospatial capabilities of carrier systems in delivering growth factors to benefit vascular bioengineering",
abstract = "In this study we produced a set of in vitro culture platforms to model vascular cell responses to growth factors and factor delivery vehicles. Two of the systems (whole vessel and whole lung vascular development) were supported by microfluidic systems facilitating media circulation and waste removal. We assessed vascular endothelial growth factor (VEGF) delivery by Pluronic F-127 hydrogel, 30 nm pore-sized microparticles (MPs), 60 nm pore-sized MP or a 50/50 mixture of 30 and 60 nm pore-sized MP. VEGF was delivered to porcine acellular lung vascular scaffolds (2.5 cm2 square pieces or whole 3D segments of acellular blood vessels) as well as whole acellular lung scaffolds. Scaffold-cell attachment was examined as was vascular tissue formation. We showed that a 50/50 mixture of 30 and 60 nm pore-sized silicon wafer MPs allowed for long-term release of VEGF within the scaffold vasculature and supported vascular endothelial tissue development during in vitro culture.",
keywords = "Acellular scaffolds, Bioengineered lung, Bioreactor culture, Microfluidic platforms, Microparticle delivery, VEGF",
author = "Argueta, {Lissenya B.} and Niles, {Jean A.} and Jason Sakamoto and Xuewu Liu and Vega, {Stephanie P.} and Luba Frank and Marco Paessler and Joaquin Cortiella and Nichols, {Joan E.}",
note = "Funding Information: We thank the animal Resource Centers and our veterinary staff for their help in acquisition of porcine lungs as part of tissue-sharing programs at UTMB, Galveston, TX, and at Houston Methodist Research Institute, Houston, TX. We would also like to thank the Kostas family for their commitment to support scientific research and discoveries in the field of cardiovascular nanomedicine. Funding: This work was supported in part by NIH U18 Grant (grant no. U18TR000560-01) and NIH NIAID Contract Number HHSN272201700040I, Task Order Number 75N93019F00131 awarded to J.E.N. Additional funding for production of nanoparticles was provided from startup funds provided to J.S. from Houston Methodist Research Institute (HMRI) of Houston, TX. The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. Funding Information: We thank the animal Resource Centers and our veterinary staff for their help in acquisition of porcine lungs as part of tissue-sharing programs at UTMB, Galveston, TX, and at Houston Methodist Research Institute, Houston, TX. We would also like to thank the Kostas family for their commitment to support scientific research and discoveries in the field of cardiovascular nanomedicine. Funding: This work was supported in part by NIH U18 Grant (grant no. U18TR000560-01 ) and NIH NIAID Contract Number HHSN272201700040I, Task Order Number 75N93019F00131 awarded to J.E.N. Additional funding for production of nanoparticles was provided from startup funds provided to J.S. from Houston Methodist Research Institute (HMRI) of Houston, TX. Publisher Copyright: {\textcopyright} 2021",
year = "2021",
month = aug,
doi = "10.1016/j.nano.2021.102419",
language = "English (US)",
volume = "36",
journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",
issn = "1549-9634",
publisher = "Elsevier",
}