@article{f606f8d9b15f4d39a044dbf6d5a40575,
title = "Systematic analysis on multiple Gene Expression Omnibus data sets reveals fierce immune response in hepatitis B virus-related acute liver failure",
abstract = "Acute liver failure (ALF) caused by hepatitis B virus (HBV) is common type of liver failure in the world, with high morbidity and mortality rates. However, the prevalence, genetic background and factors determining the development of HBV-related ALF are rarely studied. In this study, we examined three Gene Expression Omnibus (GEO) data sets by bioinformatics analysis to identify differentially expressed genes (DEGs), key biological processes and pathways. Immune infiltration analysis showed high immune cells infiltration in HBV-related ALF tissue. We then confirmed natural killer cells and macrophages infiltration in clinical samples by immunohistochemistry assay, implying these cells play a significant role in HBV-ALF. We found 1277 genes were co-up-regulated and that 1082 genes were co-down-regulated in the 3 data sets. Inflammation-related pathways were enriched in the co-up-regulated genes and synthetic metabolic pathways were enriched in the co-down-regulated genes. WGCNA also revealed a key module enriching in immune inflammation response and identified 10 hub genes, differentially expressed in an independent data set. In conclusion, we identified fierce immune inflammatory response to elucidate the immune-driven mechanism of HBV-ALF and 10 hub genes based on gene expression profiles.",
keywords = "HBV-ALF, WGCNA, bioinformatics analysis, immune, inflammation",
author = "Huadi Chen and Wenting Zhao and Yixi Zhang and Yiwen Guo and Weixin Luo and Xiaobo Wang and Yu Nie and Maodong Ye and Changjun Huang and Dongping Wang and Maogen Chen and Xiaoshun He and Qiang Zhao",
note = "Funding Information: Supported by grants as follows: National Natural Science Foundation of China (81570587, 81700557, 81401324 and 81770410), the Guangdong Provincial Key Laboratory Construction Projection on Organ Donation and Transplant Immunology (2013A061401007 and 2017B030314018), Guangdong Provincial Natural Science Funds for Major Basic Science Culture Project (2015A030308010), and Science and Technology Program of Guangzhou (201704020150), the Natural Science Foundations of Guangdong province (2016A030310141, 2020A1515010091 and 2020A1515011557) and Young Teachers Training Project of Sun Yat-sen University (K0401068). Funding Information: Supported by grants as follows: National Natural Science Foundation of China (81570587, 81700557, 81401324 and 81770410), the Guangdong Provincial Key Laboratory Construction Projection on Organ Donation and Transplant Immunology (2013A061401007 and 2017B030314018), Guangdong Provincial Natural Science Funds for Major Basic Science Culture Project (2015A030308010), and Science and Technology Program of Guangzhou (201704020150), the Natural Science Foundations of Guangdong province (2016A030310141, 2020A1515010091 and 2020A1515011557) and Young Teachers Training Project of Sun Yat‐sen University (K0401068). Publisher Copyright: {\textcopyright} 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd",
year = "2020",
month = sep,
day = "1",
doi = "10.1111/jcmm.15561",
language = "English (US)",
volume = "24",
pages = "9798--9809",
journal = "Journal of Cellular and Molecular Medicine",
issn = "1582-1838",
publisher = "Wiley",
number = "17",
}