The transcription factor Bcl11b is specifically expressed in group 2 innate lymphoid cells and is essential for their development

Yong Yu; Cui Wang; Simon Clare; Juexuan Wang; Song Choon Lee; Cordelia Brandt; Shannon Burke; Liming Lu; Daqian He; Nancy A. Jenkins; Neal G. Copeland; Gordon Dougan; Pentao Liu

doi:10.1084/jem.20142318

The transcription factor Bcl11b is specifically expressed in group 2 innate lymphoid cells and is essential for their development

Yong Yu, Cui Wang, Simon Clare, Juexuan Wang, Song Choon Lee, Cordelia Brandt, Shannon Burke, Liming Lu, Daqian He, Nancy A. Jenkins, Neal G. Copeland, Gordon Dougan, Pentao Liu

Research output: Contribution to journal › Article › peer-review

132 Scopus citations

Abstract

Group 2 innate lymphoid cells (ILCs), or ILC2s, are a subset of recently identified ILCs, which play important roles in innate immunity by producing type 2 effector cytokines. Several transcription factors have been found to have critical functions in the development of both ILC2s and T cells. We report here that Bcl11b, a transcription factor essential in T cell lineage commitment and maintenance, is specifically expressed in progenitors committed to the ILC2 lineage and is required for ILC2 development. The Bcl11b gene is expressed in ∼28% of ILC progenitors (ILCPs; common helper innate lymphoid progenitors or ILCPs expressing either ID2 or promyelocytic leukemia zinc finger, respectively). Both in vitro and in vivo, these Bcl11b-expressing early ILCPs generate only ILC2s. Inactivation of Bcl11b causes a complete loss of ILC2 development from hematopoietic progenitors, which is confirmed upon immune challenge with either papain administration or influenza virus infection.

Original language	English (US)
Pages (from-to)	865-74
Number of pages	10
Journal	The Journal of experimental medicine
Volume	212
Issue number	6
DOIs	https://doi.org/10.1084/jem.20142318
State	Published - Jun 1 2015

Keywords

Animals
Cell Lineage
Cell Separation
Chromatin Immunoprecipitation
Female
Flow Cytometry
GATA3 Transcription Factor
Gene Deletion
Gene Expression Profiling
Gene Expression Regulation
Genes, Reporter
Hematopoietic Stem Cells
Immunity, Innate
Inducible T-Cell Co-Stimulator Protein
Lymphocytes
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Receptors, Interleukin
Repressor Proteins
Tumor Suppressor Proteins
Zinc Fingers

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1084/jem.20142318

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Yu, Y., Wang, C., Clare, S., Wang, J., Lee, S. C., Brandt, C., Burke, S., Lu, L., He, D., Jenkins, N. A., Copeland, N. G., Dougan, G., & Liu, P. (2015). The transcription factor Bcl11b is specifically expressed in group 2 innate lymphoid cells and is essential for their development. The Journal of experimental medicine, 212(6), 865-74. https://doi.org/10.1084/jem.20142318

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abstract = "Group 2 innate lymphoid cells (ILCs), or ILC2s, are a subset of recently identified ILCs, which play important roles in innate immunity by producing type 2 effector cytokines. Several transcription factors have been found to have critical functions in the development of both ILC2s and T cells. We report here that Bcl11b, a transcription factor essential in T cell lineage commitment and maintenance, is specifically expressed in progenitors committed to the ILC2 lineage and is required for ILC2 development. The Bcl11b gene is expressed in ∼28% of ILC progenitors (ILCPs; common helper innate lymphoid progenitors or ILCPs expressing either ID2 or promyelocytic leukemia zinc finger, respectively). Both in vitro and in vivo, these Bcl11b-expressing early ILCPs generate only ILC2s. Inactivation of Bcl11b causes a complete loss of ILC2 development from hematopoietic progenitors, which is confirmed upon immune challenge with either papain administration or influenza virus infection.",

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author = "Yong Yu and Cui Wang and Simon Clare and Juexuan Wang and Lee, {Song Choon} and Cordelia Brandt and Shannon Burke and Liming Lu and Daqian He and Jenkins, {Nancy A.} and Copeland, {Neal G.} and Gordon Dougan and Pentao Liu",

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AU - Yu, Yong

AU - Wang, Cui

AU - Clare, Simon

AU - Wang, Juexuan

AU - Lee, Song Choon

AU - Brandt, Cordelia

AU - Burke, Shannon

AU - Lu, Liming

AU - He, Daqian

AU - Jenkins, Nancy A.

AU - Copeland, Neal G.

AU - Dougan, Gordon

AU - Liu, Pentao

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AB - Group 2 innate lymphoid cells (ILCs), or ILC2s, are a subset of recently identified ILCs, which play important roles in innate immunity by producing type 2 effector cytokines. Several transcription factors have been found to have critical functions in the development of both ILC2s and T cells. We report here that Bcl11b, a transcription factor essential in T cell lineage commitment and maintenance, is specifically expressed in progenitors committed to the ILC2 lineage and is required for ILC2 development. The Bcl11b gene is expressed in ∼28% of ILC progenitors (ILCPs; common helper innate lymphoid progenitors or ILCPs expressing either ID2 or promyelocytic leukemia zinc finger, respectively). Both in vitro and in vivo, these Bcl11b-expressing early ILCPs generate only ILC2s. Inactivation of Bcl11b causes a complete loss of ILC2 development from hematopoietic progenitors, which is confirmed upon immune challenge with either papain administration or influenza virus infection.

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KW - Mice

KW - Mice, Inbred C57BL

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ER -

The transcription factor Bcl11b is specifically expressed in group 2 innate lymphoid cells and is essential for their development

Abstract

Keywords

ASJC Scopus subject areas

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