TY - JOUR
T1 - Y-receptor-like genes GPR72 and GPR73
T2 - molecular cloning, genomic organisation and assignment to human chromosome 11q21.1 and 2p14 and mouse chromosome 9 and 6
AU - Parker, Rachel
AU - Liu, Marjorie
AU - Eyre, Helen J.
AU - Copeland, Neal G.
AU - Gilbert, Debra J.
AU - Crawford, Joanna
AU - Sutherland, Grant R.
AU - Jenkins, Nancy A.
AU - Herzog, Herbert
N1 - Funding Information:
This work was supported by the National Health and Medical Research Council of Australia and in part, by the National Cancer Institute, DHHS, under contract with ABL. We thank Deborah B. Householder and Olga Antinova for excellent technical assistance.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2000/4/25
Y1 - 2000/4/25
N2 - Two novel G-protein-coupled receptors, one from human, GPR72, and one from mouse, GPR73 have been isolated, sequenced and their genomic organisation determined. Non-isotopic in situ hybridisation and radiation hybrid mapping have identified GPR72 to be localised on human chromosome 11q21.1, and GPR73 on human chromosome 2p14. Interspecific mouse backcross mapping has localised the genes to mouse chromosomes 9 and 6, respectively. Northern analysis reveals GPR72 mRNA expression only in brain tissue. However, GPR73 mRNA can be found in heart, skeletal muscle and pancreas. Both receptors are closely related with 36 and 33% overall amino acid identity, respectively, to the Y-receptor family. However, although successful cell surface expression in a heterologous expression system can be achieved no specific binding to this ligand family can be detected, indicating that perhaps additional factors are required for binding. Copyright (C) 2000 Elsevier Science B.V.
AB - Two novel G-protein-coupled receptors, one from human, GPR72, and one from mouse, GPR73 have been isolated, sequenced and their genomic organisation determined. Non-isotopic in situ hybridisation and radiation hybrid mapping have identified GPR72 to be localised on human chromosome 11q21.1, and GPR73 on human chromosome 2p14. Interspecific mouse backcross mapping has localised the genes to mouse chromosomes 9 and 6, respectively. Northern analysis reveals GPR72 mRNA expression only in brain tissue. However, GPR73 mRNA can be found in heart, skeletal muscle and pancreas. Both receptors are closely related with 36 and 33% overall amino acid identity, respectively, to the Y-receptor family. However, although successful cell surface expression in a heterologous expression system can be achieved no specific binding to this ligand family can be detected, indicating that perhaps additional factors are required for binding. Copyright (C) 2000 Elsevier Science B.V.
KW - Cell-surface expression
KW - G-protein coupled receptor
KW - Ligand binding
KW - Neuropeptide Y
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U2 - 10.1016/S0167-4781(00)00023-3
DO - 10.1016/S0167-4781(00)00023-3
M3 - Article
C2 - 10760605
AN - SCOPUS:0034712790
SN - 0167-4781
VL - 1491
SP - 369
EP - 375
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 1-3
ER -