@article{e5139c99962e4b43b19af3a8fbcc1296,
title = "3D in vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells",
abstract = "Cornification and epidermal barrier defects are associated with a number of clinically diverse skin disorders. However, a suitable in vitro model for studying normal barrier function and barrier defects is still lacking. Here, we demonstrate the generation of human epidermal equivalents (HEEs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). HEEs are structurally similar to native epidermis, with a functional permeability barrier. We exposed a pure population of hESC/iPSC-derived keratinocytes, whose transcriptome corresponds to the gene signature of normal primary human keratinocytes (NHKs), to a sequential high-to-low humidity environment in an air/liquid interface culture. The resulting HEEs had all of the cellular strata of the human epidermis, with skin barrier properties similar to those of normal skin. Such HEEs generated from disease-specific iPSCs will be an invaluable tool not only for dissecting molecular mechanisms that lead to epidermal barrier defects but also for drug development and screening.",
author = "Anastasia Petrova and Anna Celli and Laureen Jacquet and Dimitra Dafou and Debra Crumrine and Melanie Hupe and Matthew Arno and Carl Hobbs and Aleksandra Cvoro and Panagiotis Karagiannis and Liani Devito and Richard Sun and Adame, {Lillian C.} and Robert Vaughan and McGrath, {John A.} and Mauro, {Theodora M.} and Dusko Ilic",
note = "Funding Information: For help and support, we thank H. Navsaria and G. Damodaran (Cell and Tissue Engineering, Centre for Cutaneous Research, Queen Mary, University of London), W.-L. Di (Institute of Child Health, University College London), S. Minger (GE Healthcare), and the staffs of the San Francisco Veteran Affairs Medical Center, the Genomics and Flow Cytometry facilities, and the Biomedical Research Centre at Guy{\textquoteright}s Hospital. We also thank C. Ogilvie from the Genetics Centre at Guy{\textquoteright}s Hospital for a critical reading of the manuscript. This work was supported in part by grants from the Medical Research Council UK (G0801061 to D.I.), the NIH (R21 ARO61583 and R01 AR051930), the Research Service of the Department of Veterans Affairs (to T.M.M.), and the Dystrophic Epidermolysis Bullosa Research Association (DebRA) (to J.A.M.). A.P. was a DebRA-supported PhD student. ",
year = "2014",
month = may,
day = "6",
doi = "10.1016/j.stemcr.2014.03.009",
language = "English (US)",
volume = "2",
pages = "675--689",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Elsevier",
number = "5",
}