Abstract
A simple and reproducible method is described for studying the morphology of the same neuron at the light and electron microscopic level. This method utilizes horseradish peroxidase histochemistry and a recently described flat-embedding procedure wherein thin, aldehyde-fixed sections are placed in resin between glass microscope slides pretreated with dimethyldichlorosilane. The significance of these combined methodologies for correlative light and electron microscopic studies of single neurons is discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 27-34 |
| Number of pages | 8 |
| Journal | Journal of Neuroscience Research |
| Volume | 11 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1984 |
Keywords
- HRP
- dimethyldichlorosilane
- flat‐embedding
- light and electron microscopy
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience