A flow cytometric assay of Fc receptor-mediated phagocytosis

Wolfgang H.T. Schürmann; Mark W. Frampton; Gudrun Schürmann; Norbert J. Roberts; Joan E. Nichols; Jacob N. Finkelstein; Günter Oberdörster; Mark J. Utell

doi:10.1089/jam.1997.10.1

A flow cytometric assay of Fc receptor-mediated phagocytosis

Wolfgang H.T. Schürmann, Mark W. Frampton, Gudrun Schürmann, Norbert J. Roberts, Joan E. Nichols, Jacob N. Finkelstein, Günter Oberdörster, Mark J. Utell

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A flow cytometric assay was developed that specifically measures Fc receptor-mediated phagocytosis independent of other phagocytosis-related receptors. Suspensions of rat alveolar macrophages were incubated with fluorescein isothiocyanate (FITC)labeled polystyrene microspheres and subsequently were analyzed by flow cytometry. Coating of microspheres with bovine serum albumin was used to generate 'neutral' particles demonstrating low phagocytosis by alveolar macrophages (AMs). AM attachment and uptake of these particles was increased tenfold with IgG opsonization. This increase was abolished completely by blocking of AM Fc receptors with IgG. The quenching effect of ethidium bromide (EB) on FITC fluorescence was used for differentiating attached from internalized particles. The assay was standardized and optimized for use in toxicologic studies.

Original language	English (US)
Pages (from-to)	1-8
Number of pages	8
Journal	Journal of Aerosol Medicine: Deposition, Clearance, and Effects in the Lung
Volume	10
Issue number	1
DOIs	https://doi.org/10.1089/jam.1997.10.1
State	Published - 1997

Keywords

Fc receptor
alveolar macrophages
flow cytometry
particles
phagocytosis

ASJC Scopus subject areas

Pulmonary and Respiratory Medicine
Pharmacology (medical)

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10.1089/jam.1997.10.1

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title = "A flow cytometric assay of Fc receptor-mediated phagocytosis",

abstract = "A flow cytometric assay was developed that specifically measures Fc receptor-mediated phagocytosis independent of other phagocytosis-related receptors. Suspensions of rat alveolar macrophages were incubated with fluorescein isothiocyanate (FITC)labeled polystyrene microspheres and subsequently were analyzed by flow cytometry. Coating of microspheres with bovine serum albumin was used to generate 'neutral' particles demonstrating low phagocytosis by alveolar macrophages (AMs). AM attachment and uptake of these particles was increased tenfold with IgG opsonization. This increase was abolished completely by blocking of AM Fc receptors with IgG. The quenching effect of ethidium bromide (EB) on FITC fluorescence was used for differentiating attached from internalized particles. The assay was standardized and optimized for use in toxicologic studies.",

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AU - Schürmann, Wolfgang H.T.

AU - Frampton, Mark W.

AU - Schürmann, Gudrun

AU - Roberts, Norbert J.

AU - Nichols, Joan E.

AU - Finkelstein, Jacob N.

AU - Oberdörster, Günter

AU - Utell, Mark J.

PY - 1997

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KW - flow cytometry

KW - particles

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A flow cytometric assay of Fc receptor-mediated phagocytosis

Abstract

Keywords

ASJC Scopus subject areas

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