A liaf codon deletion abolishes daptomycin bactericidal activity against vancomycin-resistant enterococcus faecalis

Jose M. Munita; Truc T. Tran; Lorena Diaz; Diana Panesso; Jinnethe Reyes; Barbara E. Murray; Cesar A. Ariasa

doi:10.1128/AAC.00021-13

A liaf codon deletion abolishes daptomycin bactericidal activity against vancomycin-resistant enterococcus faecalis

Jose M. Munita, Truc T. Tran, Lorena Diaz, Diana Panesso, Jinnethe Reyes, Barbara E. Murray, Cesar A. Ariasa

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

The genetic bases for antibiotic tolerance are obscure. Daptomycin (DAP) is a lipopeptide antibiotic with bactericidal activity against enterococci. Using time-kill assays, we provide evidence for the first time that a deletion of isoleucine in position 177 of LiaF, a member of the three-component regulatory system LiaFSR involved in the cell envelope response to antimicrobials, is directly responsible for a DAP-tolerant phenotype and is likely to negatively affect response to DAP therapy.

Original language	English (US)
Pages (from-to)	2831-2833
Number of pages	3
Journal	Antimicrobial Agents and Chemotherapy
Volume	57
Issue number	6
DOIs	https://doi.org/10.1128/AAC.00021-13
State	Published - Jun 2013

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

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abstract = "The genetic bases for antibiotic tolerance are obscure. Daptomycin (DAP) is a lipopeptide antibiotic with bactericidal activity against enterococci. Using time-kill assays, we provide evidence for the first time that a deletion of isoleucine in position 177 of LiaF, a member of the three-component regulatory system LiaFSR involved in the cell envelope response to antimicrobials, is directly responsible for a DAP-tolerant phenotype and is likely to negatively affect response to DAP therapy.",

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AU - Tran, Truc T.

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AU - Panesso, Diana

AU - Reyes, Jinnethe

AU - Murray, Barbara E.

AU - Ariasa, Cesar A.

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AB - The genetic bases for antibiotic tolerance are obscure. Daptomycin (DAP) is a lipopeptide antibiotic with bactericidal activity against enterococci. Using time-kill assays, we provide evidence for the first time that a deletion of isoleucine in position 177 of LiaF, a member of the three-component regulatory system LiaFSR involved in the cell envelope response to antimicrobials, is directly responsible for a DAP-tolerant phenotype and is likely to negatively affect response to DAP therapy.

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A liaf codon deletion abolishes daptomycin bactericidal activity against vancomycin-resistant enterococcus faecalis

Abstract

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