A microfluidic platform for the ultrasensitive detection of human enterovirus 71

Xuejiao Guan; Feng Wu; Mao Mao; Lei Li; Jie Sun; Dan Liu; Zhifeng Qin; Jikui Deng; Xueying Shi; Lan Ma

doi:10.1016/j.snr.2021.100046

A microfluidic platform for the ultrasensitive detection of human enterovirus 71

Xuejiao Guan, Feng Wu, Mao Mao, Lei Li, Jie Sun, Dan Liu, Zhifeng Qin, Jikui Deng, Xueying Shi, Lan Ma

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A microfluidic platform integrated with two generated monoclonal antibodies (mAbs) was innovatively fabricated for ultrasensitive detection of enterovirus 71 (EV71). To date, this is the first report on EV71 detection using a microfluidic chip-based immunoassay. The mAbs 1F4 and 2H2 against the major capsid protein VP1 of EV71 were conjugated with carboxyl-functionalized magnetic beads (MBs) and carboxyl-functionalized quantum dots (QDs), respectively. MB-conjugated mAbs separated VP1 from samples, while QD-conjugated mAbs provided fluorescence detection signals and formed a sandwich structure. The monolayer of MBs and QDs captured the VP1 sandwich structure showed stronger fluorescence signal in the microfluidic channel, which could be directly evaluated by fluorescence intensity in response to 365 nm ultraviolet (UV) excitation. The limit of detection (LOD) of the microfluidic platform for VP1 was 10 pg mL⁻¹, which was a 31-fold increase compared to sandwich enzyme-linked immunosorbent assay (ELISA, 310 pg mL⁻¹) using identical antibodies. The microfluidic platform performed well with clinical samples, especially for fecal samples with complex compositions and impurities. The assay is quick to perform (within 30 min), and apparently requires just one syringe without other complex liquid flow system, making it is easy to operate and more suitable for on-site detection and future commercial application.

Original language	English (US)
Article number	100046
Journal	Sensors and Actuators Reports
Volume	3
DOIs	https://doi.org/10.1016/j.snr.2021.100046
State	Published - Nov 2021

Keywords

EV71
Microfluidic immunoassay, Ultrasensitive detection
Microfluidic platform

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Computer Science (miscellaneous)
Atomic and Molecular Physics, and Optics
Instrumentation
Electrical and Electronic Engineering

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10.1016/j.snr.2021.100046

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title = "A microfluidic platform for the ultrasensitive detection of human enterovirus 71",

abstract = "A microfluidic platform integrated with two generated monoclonal antibodies (mAbs) was innovatively fabricated for ultrasensitive detection of enterovirus 71 (EV71). To date, this is the first report on EV71 detection using a microfluidic chip-based immunoassay. The mAbs 1F4 and 2H2 against the major capsid protein VP1 of EV71 were conjugated with carboxyl-functionalized magnetic beads (MBs) and carboxyl-functionalized quantum dots (QDs), respectively. MB-conjugated mAbs separated VP1 from samples, while QD-conjugated mAbs provided fluorescence detection signals and formed a sandwich structure. The monolayer of MBs and QDs captured the VP1 sandwich structure showed stronger fluorescence signal in the microfluidic channel, which could be directly evaluated by fluorescence intensity in response to 365 nm ultraviolet (UV) excitation. The limit of detection (LOD) of the microfluidic platform for VP1 was 10 pg mL−1, which was a 31-fold increase compared to sandwich enzyme-linked immunosorbent assay (ELISA, 310 pg mL−1) using identical antibodies. The microfluidic platform performed well with clinical samples, especially for fecal samples with complex compositions and impurities. The assay is quick to perform (within 30 min), and apparently requires just one syringe without other complex liquid flow system, making it is easy to operate and more suitable for on-site detection and future commercial application.",

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author = "Xuejiao Guan and Feng Wu and Mao Mao and Lei Li and Jie Sun and Dan Liu and Zhifeng Qin and Jikui Deng and Xueying Shi and Lan Ma",

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AU - Guan, Xuejiao

AU - Wu, Feng

AU - Mao, Mao

AU - Li, Lei

AU - Sun, Jie

AU - Liu, Dan

AU - Qin, Zhifeng

AU - Deng, Jikui

AU - Shi, Xueying

AU - Ma, Lan

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AB - A microfluidic platform integrated with two generated monoclonal antibodies (mAbs) was innovatively fabricated for ultrasensitive detection of enterovirus 71 (EV71). To date, this is the first report on EV71 detection using a microfluidic chip-based immunoassay. The mAbs 1F4 and 2H2 against the major capsid protein VP1 of EV71 were conjugated with carboxyl-functionalized magnetic beads (MBs) and carboxyl-functionalized quantum dots (QDs), respectively. MB-conjugated mAbs separated VP1 from samples, while QD-conjugated mAbs provided fluorescence detection signals and formed a sandwich structure. The monolayer of MBs and QDs captured the VP1 sandwich structure showed stronger fluorescence signal in the microfluidic channel, which could be directly evaluated by fluorescence intensity in response to 365 nm ultraviolet (UV) excitation. The limit of detection (LOD) of the microfluidic platform for VP1 was 10 pg mL−1, which was a 31-fold increase compared to sandwich enzyme-linked immunosorbent assay (ELISA, 310 pg mL−1) using identical antibodies. The microfluidic platform performed well with clinical samples, especially for fecal samples with complex compositions and impurities. The assay is quick to perform (within 30 min), and apparently requires just one syringe without other complex liquid flow system, making it is easy to operate and more suitable for on-site detection and future commercial application.

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A microfluidic platform for the ultrasensitive detection of human enterovirus 71

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