TY - JOUR
T1 - A novel virus-encoded nucleocytoplasmic shuttling protein
T2 - The UL3 protein of herpes simplex virus type 1
AU - Zheng, Chunfu
AU - Lin, Fusen
AU - Wang, Shuai
AU - Xing, Junji
N1 - Funding Information:
We are grateful to Dr. Yoshinari Yasuda for providing pGEX-6p-1 Q69L Ran and Dr. Gillian Elliott for the generous gift plasmid pRev-NES-EGFP. This research was supported by the Major State Basic Research Development Program of China (973 Program) ( 2010CB530105 and 2011CB504802 ); the Start-up Fund of the Hundred Talents Program of the Chinese Academy of Sciences ( 20071010-141 ) and the National Natural Science Foundation of China ( 30870120 , 30900059 and 81000736 ).
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011/11
Y1 - 2011/11
N2 - Herpes simplex virus type 1 (HSV-1) UL3 protein is a nuclear protein. In this study, the molecular mechanism of the subcellular localization of UL3 was characterized by fluorescence microscopy in living cells. A nuclear localization signal (NLS) and a nuclear export signal (NES) were also identified. UL3 was demonstrated to target to the cytoplasm through the NES via chromosomal region maintenance 1 (CRM-1) dependent pathway, and to the nucleus through RanGTP-dependent mechanism. Heterokaryon assays confirmed that UL3 was capable of shuttling between the nucleus and the cytoplasm. These results demonstrate that the UL3 protein is a novel HSV-1 encoded nucleocytoplasmic shuttling protein.
AB - Herpes simplex virus type 1 (HSV-1) UL3 protein is a nuclear protein. In this study, the molecular mechanism of the subcellular localization of UL3 was characterized by fluorescence microscopy in living cells. A nuclear localization signal (NLS) and a nuclear export signal (NES) were also identified. UL3 was demonstrated to target to the cytoplasm through the NES via chromosomal region maintenance 1 (CRM-1) dependent pathway, and to the nucleus through RanGTP-dependent mechanism. Heterokaryon assays confirmed that UL3 was capable of shuttling between the nucleus and the cytoplasm. These results demonstrate that the UL3 protein is a novel HSV-1 encoded nucleocytoplasmic shuttling protein.
KW - Chromosomal region maintenance 1
KW - HSV-1
KW - Nuclear export signal
KW - Nuclear localization signal
KW - Ran-GTP
KW - UL3
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U2 - 10.1016/j.jviromet.2011.08.004
DO - 10.1016/j.jviromet.2011.08.004
M3 - Article
C2 - 21864580
AN - SCOPUS:80053131428
SN - 0166-0934
VL - 177
SP - 206
EP - 210
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 2
ER -