A Plaque Method for Titration of Frog Viruses Using Starch Gel Overlay

Daniel E. Lehane; H. Fred Clark; David T. Karzon

doi:10.3181/00379727-125-32010

A Plaque Method for Titration of Frog Viruses Using Starch Gel Overlay

Daniel E. Lehane, H. Fred Clark, David T. Karzon

Department of Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A reliable plaque assay system has been developed for several recently isolated frog viruses. Cell lines from reptiles, amphibians, and fish were tested for their ability to be maintained under various solid overlay materials. Starch gel was found to be the most useful overlay material. The most sensitive assay system was shown to be Terrapene heart (TH-1) cell culture.

Original language	English (US)
Pages (from-to)	50-54
Number of pages	5
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	125
Issue number	1
DOIs	https://doi.org/10.3181/00379727-125-32010
State	Published - May 1967

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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title = "A Plaque Method for Titration of Frog Viruses Using Starch Gel Overlay",

abstract = "A reliable plaque assay system has been developed for several recently isolated frog viruses. Cell lines from reptiles, amphibians, and fish were tested for their ability to be maintained under various solid overlay materials. Starch gel was found to be the most useful overlay material. The most sensitive assay system was shown to be Terrapene heart (TH-1) cell culture.",

author = "Lehane, {Daniel E.} and Clark, {H. Fred} and Karzon, {David T.}",

note = "Funding Information: Supported in part by USPHS Research Grants AI-02396 and CA-08737, Training Grant AI-98, and General Research Support Grant FR-05400. ",

year = "1967",

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T1 - A Plaque Method for Titration of Frog Viruses Using Starch Gel Overlay

AU - Lehane, Daniel E.

AU - Clark, H. Fred

AU - Karzon, David T.

N1 - Funding Information: Supported in part by USPHS Research Grants AI-02396 and CA-08737, Training Grant AI-98, and General Research Support Grant FR-05400.

PY - 1967/5

Y1 - 1967/5

N2 - A reliable plaque assay system has been developed for several recently isolated frog viruses. Cell lines from reptiles, amphibians, and fish were tested for their ability to be maintained under various solid overlay materials. Starch gel was found to be the most useful overlay material. The most sensitive assay system was shown to be Terrapene heart (TH-1) cell culture.

AB - A reliable plaque assay system has been developed for several recently isolated frog viruses. Cell lines from reptiles, amphibians, and fish were tested for their ability to be maintained under various solid overlay materials. Starch gel was found to be the most useful overlay material. The most sensitive assay system was shown to be Terrapene heart (TH-1) cell culture.

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A Plaque Method for Titration of Frog Viruses Using Starch Gel Overlay

Abstract

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