A preliminary investigation of Ehrlichia species in ticks, humans, dogs, and capybaras from Brazil

Marcelo B. Labruna; Jere W. McBride; Luis Marcelo A. Camargo; Daniel M. Aguiar; Michael J. Yabsley; William R. Davidson; Ellen Y. Stromdahl; Phillip C. Williamson; Roger W. Stich; S. Wesley Long; Erney P. Camargo; David H. Walker

doi:10.1016/j.vetpar.2006.08.005

A preliminary investigation of Ehrlichia species in ticks, humans, dogs, and capybaras from Brazil

Marcelo B. Labruna, Jere W. McBride, Luis Marcelo A. Camargo, Daniel M. Aguiar, Michael J. Yabsley, William R. Davidson, Ellen Y. Stromdahl, Phillip C. Williamson, Roger W. Stich, S. Wesley Long, Erney P. Camargo, David H. Walker

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

A molecular epidemiologic investigation in two Brazilian states (Rondônia and São Paulo) was undertaken to determine if Ehrlichia species responsible for human and animal ehrlichioses in North America could be found in Brazilian vectors, potential natural mammalian reservoirs and febrile human patients with a tick bite history. Samples, including 376 ticks comprising 9 Amblyomma species, 29 capybara (Hydrochaeris hydrochaeris) spleens, 5 canine blood, and 75 human blood samples from febrile patients with history of tick bites were tested by a real-time PCR assay targeting a fragment of the Ehrlichia dsb gene. Ehrlichia DNA was not detected in any tick, capybara or human samples. In contrast, 4 out of 5 dogs contained Ehrlichia canis DNA in their blood, which were sequenced, representing the first report of E. canis infecting dogs in the Amazon region of Brazil. Further studies are needed to evaluate the presence of other agents of human and animal ehrlichioses in Brazil.

Original language	English (US)
Pages (from-to)	189-195
Number of pages	7
Journal	Veterinary Parasitology
Volume	143
Issue number	2
DOIs	https://doi.org/10.1016/j.vetpar.2006.08.005
State	Published - Jan 31 2007

Keywords

Brazil
Dsb gene
Ehrlichia
Real-time PCR
Ticks

ASJC Scopus subject areas

Animal Science and Zoology
Parasitology
veterinary(all)

Access to Document

10.1016/j.vetpar.2006.08.005

Cite this

Labruna, M. B., McBride, J. W., Camargo, L. M. A., Aguiar, D. M., Yabsley, M. J., Davidson, W. R., Stromdahl, E. Y., Williamson, P. C., Stich, R. W., Long, S. W., Camargo, E. P., & Walker, D. H. (2007). A preliminary investigation of Ehrlichia species in ticks, humans, dogs, and capybaras from Brazil. Veterinary Parasitology, 143(2), 189-195. https://doi.org/10.1016/j.vetpar.2006.08.005

@article{ba03a2f06fe54141bb6da8a3e2d56e68,

title = "A preliminary investigation of Ehrlichia species in ticks, humans, dogs, and capybaras from Brazil",

abstract = "A molecular epidemiologic investigation in two Brazilian states (Rond{\^o}nia and S{\~a}o Paulo) was undertaken to determine if Ehrlichia species responsible for human and animal ehrlichioses in North America could be found in Brazilian vectors, potential natural mammalian reservoirs and febrile human patients with a tick bite history. Samples, including 376 ticks comprising 9 Amblyomma species, 29 capybara (Hydrochaeris hydrochaeris) spleens, 5 canine blood, and 75 human blood samples from febrile patients with history of tick bites were tested by a real-time PCR assay targeting a fragment of the Ehrlichia dsb gene. Ehrlichia DNA was not detected in any tick, capybara or human samples. In contrast, 4 out of 5 dogs contained Ehrlichia canis DNA in their blood, which were sequenced, representing the first report of E. canis infecting dogs in the Amazon region of Brazil. Further studies are needed to evaluate the presence of other agents of human and animal ehrlichioses in Brazil.",

keywords = "Brazil, Dsb gene, Ehrlichia, Real-time PCR, Ticks",

author = "Labruna, {Marcelo B.} and McBride, {Jere W.} and Camargo, {Luis Marcelo A.} and Aguiar, {Daniel M.} and Yabsley, {Michael J.} and Davidson, {William R.} and Stromdahl, {Ellen Y.} and Williamson, {Phillip C.} and Stich, {Roger W.} and Long, {S. Wesley} and Camargo, {Erney P.} and Walker, {David H.}",

note = "Funding Information: We thank R.E. Corstvet for providing A. platys-infected canine platelets, and J.S. Dumler for providing the A. phagocytophilum-infected cells. This work was supported by Fogarty International Center (grant D43TW00903 to DHW and MBL), Funda{\c c}{\~a}o de Amparo {\`a} Pesquisa do Estado de S{\~a}o Paulo, Brazil (grant 02/00644-0 to MBL), National Institutes of Allergy and Infectious Diseases (grant R01 AI044235-2 to WRD).",

year = "2007",

month = jan,

day = "31",

doi = "10.1016/j.vetpar.2006.08.005",

language = "English (US)",

volume = "143",

pages = "189--195",

journal = "Veterinary Parasitology",

issn = "0304-4017",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - A preliminary investigation of Ehrlichia species in ticks, humans, dogs, and capybaras from Brazil

AU - Labruna, Marcelo B.

AU - McBride, Jere W.

AU - Camargo, Luis Marcelo A.

AU - Aguiar, Daniel M.

AU - Yabsley, Michael J.

AU - Davidson, William R.

AU - Stromdahl, Ellen Y.

AU - Williamson, Phillip C.

AU - Stich, Roger W.

AU - Long, S. Wesley

AU - Camargo, Erney P.

AU - Walker, David H.

N1 - Funding Information: We thank R.E. Corstvet for providing A. platys-infected canine platelets, and J.S. Dumler for providing the A. phagocytophilum-infected cells. This work was supported by Fogarty International Center (grant D43TW00903 to DHW and MBL), Fundação de Amparo à Pesquisa do Estado de São Paulo, Brazil (grant 02/00644-0 to MBL), National Institutes of Allergy and Infectious Diseases (grant R01 AI044235-2 to WRD).

PY - 2007/1/31

Y1 - 2007/1/31

N2 - A molecular epidemiologic investigation in two Brazilian states (Rondônia and São Paulo) was undertaken to determine if Ehrlichia species responsible for human and animal ehrlichioses in North America could be found in Brazilian vectors, potential natural mammalian reservoirs and febrile human patients with a tick bite history. Samples, including 376 ticks comprising 9 Amblyomma species, 29 capybara (Hydrochaeris hydrochaeris) spleens, 5 canine blood, and 75 human blood samples from febrile patients with history of tick bites were tested by a real-time PCR assay targeting a fragment of the Ehrlichia dsb gene. Ehrlichia DNA was not detected in any tick, capybara or human samples. In contrast, 4 out of 5 dogs contained Ehrlichia canis DNA in their blood, which were sequenced, representing the first report of E. canis infecting dogs in the Amazon region of Brazil. Further studies are needed to evaluate the presence of other agents of human and animal ehrlichioses in Brazil.

AB - A molecular epidemiologic investigation in two Brazilian states (Rondônia and São Paulo) was undertaken to determine if Ehrlichia species responsible for human and animal ehrlichioses in North America could be found in Brazilian vectors, potential natural mammalian reservoirs and febrile human patients with a tick bite history. Samples, including 376 ticks comprising 9 Amblyomma species, 29 capybara (Hydrochaeris hydrochaeris) spleens, 5 canine blood, and 75 human blood samples from febrile patients with history of tick bites were tested by a real-time PCR assay targeting a fragment of the Ehrlichia dsb gene. Ehrlichia DNA was not detected in any tick, capybara or human samples. In contrast, 4 out of 5 dogs contained Ehrlichia canis DNA in their blood, which were sequenced, representing the first report of E. canis infecting dogs in the Amazon region of Brazil. Further studies are needed to evaluate the presence of other agents of human and animal ehrlichioses in Brazil.

KW - Brazil

KW - Dsb gene

KW - Ehrlichia

KW - Real-time PCR

KW - Ticks

UR - http://www.scopus.com/inward/record.url?scp=33845874683&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845874683&partnerID=8YFLogxK

U2 - 10.1016/j.vetpar.2006.08.005

DO - 10.1016/j.vetpar.2006.08.005

M3 - Article

C2 - 16962245

AN - SCOPUS:33845874683

SN - 0304-4017

VL - 143

SP - 189

EP - 195

JO - Veterinary Parasitology

JF - Veterinary Parasitology

IS - 2

ER -

A preliminary investigation of Ehrlichia species in ticks, humans, dogs, and capybaras from Brazil

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this