A single-dose F1-based mRNA-LNP vaccine provides protection against the lethal plague bacterium

Edo Kon; Yinon Levy; Uri Elia; Hila Cohen; Inbal Hazan-Halevy; Moshe Aftalion; Assaf Ezra; Erez Bar-Haim; Gonna Somu Naidu; Yael Diesendruck; Shahar Rotem; Nitay Ad-El; Meir Goldsmith; Emanuelle Mamroud; Dan Peer; Ofer Cohen

doi:10.1126/sciadv.adg1036

A single-dose F1-based mRNA-LNP vaccine provides protection against the lethal plague bacterium

Edo Kon, Yinon Levy, Uri Elia, Hila Cohen, Inbal Hazan-Halevy, Moshe Aftalion, Assaf Ezra, Erez Bar-Haim, Gonna Somu Naidu, Yael Diesendruck, Shahar Rotem, Nitay Ad-El, Meir Goldsmith, Emanuelle Mamroud, Dan Peer, Ofer Cohen

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Messenger RNA (mRNA) lipid nanoparticle (LNP) vaccines have emerged as an effective vaccination strategy. Although currently applied toward viral pathogens, data concerning the platform’s effectiveness against bacterial pathogens are limited. Here, we developed an effective mRNA-LNP vaccine against a lethal bacterial pathogen by optimizing mRNA payload guanine and cytosine content and antigen design. We designed a nucleoside-modified mRNA-LNP vaccine based on the bacterial F1 capsule antigen, a major protective component of Yersinia pestis, the etiological agent of plague. Plague is a rapidly deteriorating contagious disease that has killed millions of people during the history of humankind. Now, the disease is treated effectively with antibiotics; however, in the case of a multiple-antibiotic-resistant strain outbreak, alternative countermeasures are required. Our mRNA-LNP vaccine elicited humoral and cellular immunological responses in C57BL/6 mice and conferred rapid, full protection against lethal Y. pestis infection after a single dose. These data open avenues for urgently needed effective antibacterial vaccines.

Original language	English (US)
Article number	eadg1036
Pages (from-to)	eadg1036
Journal	Science advances
Volume	9
Issue number	10
DOIs	https://doi.org/10.1126/sciadv.adg1036
State	Published - Mar 10 2023

Keywords

Animals
Antigens, Bacterial/genetics
Bacterial Proteins/genetics
Mice
Mice, Inbred C57BL
Plague Vaccine/genetics
Plague/prevention & control
Yersinia pestis/genetics

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10.1126/sciadv.adg1036

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Kon, E., Levy, Y., Elia, U., Cohen, H., Hazan-Halevy, I., Aftalion, M., Ezra, A., Bar-Haim, E., Naidu, G. S., Diesendruck, Y., Rotem, S., Ad-El, N., Goldsmith, M., Mamroud, E., Peer, D., & Cohen, O. (2023). A single-dose F1-based mRNA-LNP vaccine provides protection against the lethal plague bacterium. Science advances, 9(10), eadg1036. Article eadg1036. https://doi.org/10.1126/sciadv.adg1036

Kon, E, Levy, Y, Elia, U, Cohen, H, Hazan-Halevy, I, Aftalion, M, Ezra, A, Bar-Haim, E, Naidu, GS, Diesendruck, Y, Rotem, S, Ad-El, N, Goldsmith, M, Mamroud, E, Peer, D & Cohen, O 2023, 'A single-dose F1-based mRNA-LNP vaccine provides protection against the lethal plague bacterium', Science advances, vol. 9, no. 10, eadg1036, pp. eadg1036. https://doi.org/10.1126/sciadv.adg1036

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A single-dose F1-based mRNA-LNP vaccine provides protection against the lethal plague bacterium

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