TY - JOUR
T1 - Acetonitrile-based solvents
T2 - Their application in thin-layer chromatography of cyclic nucleotides, nucleosides, purine, and pyrimidines
AU - Tomashefski, Joseph F.
AU - Barrios, Roberto J.
AU - Sudilovsky, Oscar
N1 - Funding Information:
1 This work was supported in part by Smerican Cancer Society Grant and by Case Western Reserve University Student Fellowship (to J.F.T.). * Present address: Tecoyatitla 281, Mexico 20, D. F. Mexico. a To whom reprint requests should be made. ’ Abbreviations : 3’,5’-cyclic AMP = adenosine 3’,5’-monophosphate cyclic AMP = N”-2’-0-Dibutyryl cyclic 3’,5’-adenosine monophosphate GMP = guanosine 3’,5’-monophosphate. Abbreviations for nucleotides mended by the IUPAC-IUB Commission on Biochemical Nomenclature Biophys. Acta 108: (1965) I).
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1974/8
Y1 - 1974/8
N2 - Acetonitrile-based solvent mixtures have been applied to the separation of nucleotides, nucleosides, purines, pyrimidines, and cyclic nucleotides by thin-layer chromatography. The Rf's of over 35 compounds are presented. Development times with some of the systems were as low as 16 min. The use of acetonitrile-containing solvents in adenyl cyclase and cyclic phosphodiesterase assays and in the nucleotides and nucleic acid fields is discussed.
AB - Acetonitrile-based solvent mixtures have been applied to the separation of nucleotides, nucleosides, purines, pyrimidines, and cyclic nucleotides by thin-layer chromatography. The Rf's of over 35 compounds are presented. Development times with some of the systems were as low as 16 min. The use of acetonitrile-containing solvents in adenyl cyclase and cyclic phosphodiesterase assays and in the nucleotides and nucleic acid fields is discussed.
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U2 - 10.1016/0003-2697(74)90271-1
DO - 10.1016/0003-2697(74)90271-1
M3 - Article
C2 - 4367537
AN - SCOPUS:0016205430
SN - 0003-2697
VL - 60
SP - 589
EP - 595
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -