@article{91407c701f574c6e9b8928bf98659723,
title = "Addicting diverse bacteria to a noncanonical amino acid",
abstract = "Engineered orthogonal translation systems have greatly enabled the expansion of the genetic code using noncanonical amino acids (NCAAs). However, the impact of NCAAs on organismal evolution remains unclear, in part because it is difficult to force the adoption of new genetic codes in organisms. By reengineering TEM-1 β-lactamase to be dependent on a NCAA, we maintained bacterial NCAA dependence for hundreds of generations without escape.",
author = "Tack, {Drew S.} and Ellefson, {Jared W.} and Ross Thyer and Bo Wang and Jimmy Gollihar and Forster, {Matthew T.} and Ellington, {Andrew D.}",
note = "Funding Information: This work was supported by the following grants: National Security Science and Engineering Faculty Fellowship grant FA9550-10-1-0169 to A.D.E. Welch Foundation grant F-1654. Defense Advanced Research Projects Agency N66001-14-2-4051 to A.D.E. Air Force Office of Scientific Research grant FA9550-14-1-0089 to A.D.E. Defense Advanced Research Projects Agency HR0011-15-C0095 to A.D.E. Publisher Copyright: {\textcopyright} 2016 Nature America, Inc. All rights reserved.",
year = "2016",
month = feb,
day = "1",
doi = "10.1038/nchembio.2002",
language = "English (US)",
volume = "12",
pages = "138--140",
journal = "Nature Chemical Biology",
issn = "1552-4450",
publisher = "Nature Publishing Group",
number = "3",
}