TY - JOUR
T1 - Analysis of genetic diversity and population structure of Magnaporthe grisea, the causal agent of foxtail millet blast using microsatellites
AU - Dhivya, Manimozhi
AU - Senthilraja, Govindasamy
AU - Tharmalingam, Nagendran
AU - Harish, Sankarasubramanian
AU - Saravanakumari, Kalaiselvan
AU - Anand, Theerthagiri
AU - Thiruvudainambi, Sundararajan
N1 - Funding Information:
The research project was funded by the Science and Engineering Research Board (SERB), Department of Science and Technology (DST), New Delhi, India (ECR/2016/000982). P20GM121344 from the National Institutes of Health and the National Institute of General Medical Sciences award to COBRE-CARTD (Center for Antimicrobial Resistance and Therapeutic Discovery) supports Nagendran Tharmalingam via a pilot grant. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Funding Information:
The research project was funded by the Science and Engineering Research Board (SERB), Department of Science and Technology (DST), New Delhi, India (ECR/2016/000982). P20GM121344 from the National Institutes of Health and the National Institute of General Medical Sciences award to COBRE- CARTD (Center for Antimicrobial Resistance and Therapeutic Discovery) supports Nagendran Tharmalingam via a pilot grant. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Funding Information:
The following grant information was disclosed by the authors: Science and Engineering Research Board (SERB), Department of Science and Technology (DST), New Delhi, India: ECR/2016/000982. National Institutes of Health and the National Institute of General Medical Sciences: P20GM121344.
Publisher Copyright:
© Copyright 2023 Dhivya et al.
PY - 2023/11
Y1 - 2023/11
N2 - Foxtail millet blast caused by Magnaporthe grisea is becoming a severe problem in foxtail millet growing regions of India. The genetic diversity and population structure of foxtail millet infecting M. grisea is crucial for developing effective management strategies, such as breeding blast-resistant cultivars. We analyzed thirty-two M. grisea isolates from ten foxtail millet-growing districts in Tamil Nadu, India for genetic diversity using twenty-nine microsatellite or simple sequence repeat (SSR) markers. A total of 103 alleles were identified with a mean of 3.55 alleles/locus. Gene diversity ranged from 0.170 to 0.717, while major allelic frequencies ranged from 0.344 to 0.906. The polymorphism information content (PIC) ranged from 0.155 to 0.680, with a mean value of 0.465. Population structure analysis of the genomic data sets revealed two major populations (SP1 and SP2) with different levels of ancestral admixture among the 32 blast isolates. Phylogenetic analysis classified the isolates into three major clusters. Analysis of molecular variance (AMOVA) showed high genetic variation among individuals and less among populations. Principal Coordinate Analysis (PCoA) revealed 27.16% genetic variation among populations. The present study provides the first report on the genetic diversity and population structure of the foxtail millet-infecting M. grisea population in Tamil Nadu, which could be useful for the development of blast-resistant foxtail millet cultivars.
AB - Foxtail millet blast caused by Magnaporthe grisea is becoming a severe problem in foxtail millet growing regions of India. The genetic diversity and population structure of foxtail millet infecting M. grisea is crucial for developing effective management strategies, such as breeding blast-resistant cultivars. We analyzed thirty-two M. grisea isolates from ten foxtail millet-growing districts in Tamil Nadu, India for genetic diversity using twenty-nine microsatellite or simple sequence repeat (SSR) markers. A total of 103 alleles were identified with a mean of 3.55 alleles/locus. Gene diversity ranged from 0.170 to 0.717, while major allelic frequencies ranged from 0.344 to 0.906. The polymorphism information content (PIC) ranged from 0.155 to 0.680, with a mean value of 0.465. Population structure analysis of the genomic data sets revealed two major populations (SP1 and SP2) with different levels of ancestral admixture among the 32 blast isolates. Phylogenetic analysis classified the isolates into three major clusters. Analysis of molecular variance (AMOVA) showed high genetic variation among individuals and less among populations. Principal Coordinate Analysis (PCoA) revealed 27.16% genetic variation among populations. The present study provides the first report on the genetic diversity and population structure of the foxtail millet-infecting M. grisea population in Tamil Nadu, which could be useful for the development of blast-resistant foxtail millet cultivars.
KW - Blast
KW - Diversity
KW - Foxtail millet
KW - Microsatellites
KW - Phylogenetics
KW - Population structure
KW - India/epidemiology
KW - Humans
KW - Plant Breeding
KW - Setaria Plant/genetics
KW - Phylogeny
KW - Polymorphism, Genetic/genetics
KW - Microsatellite Repeats/genetics
KW - Pyricularia grisea/genetics
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UR - http://www.scopus.com/inward/citedby.url?scp=85177209178&partnerID=8YFLogxK
U2 - 10.7717/PEERJ.16258
DO - 10.7717/PEERJ.16258
M3 - Article
C2 - 37927781
AN - SCOPUS:85177209178
SN - 2167-8359
VL - 11
SP - e16258
JO - PeerJ
JF - PeerJ
M1 - e16258
ER -