Abstract
S49 are mouse thymic lymphoma cells which do not express the two closely linked mouse metallothionein (MT) genes; however, previous studies demonstrated that treatment of S49 cells with chemical carcinogens or ultraviolet irradiation can activate these quiescent genes. To determine if activation of MT-I or MT-II in these variants is a result of cis-acting mutations, we amplified and sequenced the immediate 5' regions of 19 cadmium resistant S49 variants: MT-I+/MT-II-, MT-I-/MT-II+, MT-I+/MT-II+, and MT-I-/MT-II-. None of the variants contained mutations in the analyzed regions. Thus, the observed changes in MT expression must result from mutations at other sites or from non-mutational mechanisms.
Original language | English (US) |
---|---|
Pages (from-to) | 217-221 |
Number of pages | 5 |
Journal | Gene expression |
Volume | 1 |
Issue number | 3 |
State | Published - 1991 |
ASJC Scopus subject areas
- Molecular Biology
- Genetics