TY - JOUR
T1 - Antigenic relationships among frog viruses demonstrated by the plaque reduction and neutralization kinetics tests
AU - Lehane, Daniel E.
AU - Clark, H. Fred
AU - Karzon, David T.
N1 - Funding Information:
1 Supported in part by research grant AI-02396 and t,raining grant AI-98 from the National Institute of Allergy and Infectious Diseases, Research grant CA-08737 from the National Cancer Institute, and USPHS General Research Support Grant FR-05400. 2 Present address: Department of Medicine, Buffalo General Hospital, 100 High Street, Buffalo, New York, 14203. 3 Recipient of Research Career 1136 from the National Institute Infectious Diseases.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1968/4
Y1 - 1968/4
N2 - Antisera to several different cytoplasmic frog virus isolates were prepared in rabbits. Comparison of eight virus isolates by means of 50% plaque reduction tests revealed no antigenic differences. In neutralization kinetic studies of three isolates, LT-1, LT-2, and FV-1, very low neutralization rate constants (K = 0.18-2.9) and high persistent fractions of nonneutralizable virus (14-38%) were obtained. No antigenic differences were detected, although one isolate, LT-2, consistently gave lower K values and higher persistent fractions with homologous and heterologous sera than did LT-1 and FV-1. It is concluded that all the cytoplasmic frog viruses studied, originating from normal and tumor-bearing frogs from widely separated parts of the United States, comprise a single serotype.
AB - Antisera to several different cytoplasmic frog virus isolates were prepared in rabbits. Comparison of eight virus isolates by means of 50% plaque reduction tests revealed no antigenic differences. In neutralization kinetic studies of three isolates, LT-1, LT-2, and FV-1, very low neutralization rate constants (K = 0.18-2.9) and high persistent fractions of nonneutralizable virus (14-38%) were obtained. No antigenic differences were detected, although one isolate, LT-2, consistently gave lower K values and higher persistent fractions with homologous and heterologous sera than did LT-1 and FV-1. It is concluded that all the cytoplasmic frog viruses studied, originating from normal and tumor-bearing frogs from widely separated parts of the United States, comprise a single serotype.
UR - http://www.scopus.com/inward/record.url?scp=0014277150&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0014277150&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(68)90080-9
DO - 10.1016/0042-6822(68)90080-9
M3 - Article
C2 - 4297121
AN - SCOPUS:0014277150
SN - 0042-6822
VL - 34
SP - 590
EP - 595
JO - Virology
JF - Virology
IS - 4
ER -