Antiviral activities of ISG20 in positive-strand RNA virus infections

Zhi Zhou; Nan Wang; Sara E. Woodson; Qingming Dong; Jie Wang; Yuqiong Liang; Rene Rijnbrand; Lai Wei; Joan E. Nichols; Ju Tao Guo; Michael R. Holbrook; Stanley M. Lemon; Kui Li

doi:10.1016/j.virol.2010.10.008

Antiviral activities of ISG20 in positive-strand RNA virus infections

Zhi Zhou, Nan Wang, Sara E. Woodson, Qingming Dong, Jie Wang, Yuqiong Liang, Rene Rijnbrand, Lai Wei, Joan E. Nichols, Ju Tao Guo, Michael R. Holbrook, Stanley M. Lemon, Kui Li

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

ISG20 is an interferon-inducible 3-5exonuclease that inhibits replication of several human and animal RNA viruses. However, the specificities of ISG20's antiviral action remain poorly defined. Here we determine the impact of ectopic expression of ISG20 on replication of several positive-strand RNA viruses from distinct viral families. ISG20 inhibited infections by cell culture-derived hepatitis C virus (HCV) and a pestivirus, bovine viral diarrhea virus and a picornavirus, hepatitis A virus. Moreover, ISG20 demonstrated cell-type specific antiviral activity against yellow fever virus, a classical flavivirus. Overexpression of ISG20, however, did not inhibit propagation of severe acute respiratory syndrome coronavirus, a highly-pathogenic human coronavirus in Huh7.5 cells. The antiviral effects of ISG20 were all dependent on its exonuclease activity. The closely related cellular exonucleases, ISG20L1 and ISG20L2, did not inhibit HCV replication. Together, these data may help better understand the antiviral specificity and action of ISG20.

Original language	English (US)
Pages (from-to)	175-188
Number of pages	14
Journal	Virology
Volume	409
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2010.10.008
State	Published - Jan 20 2011

Keywords

Antiviral
Bovine viral diarrhea virus
Hepatitis A virus
Hepatitis C virus
ISG20
ISG20L1
ISG20L2
Innate immunity
Interferon
Severe acute respiratory syndrome coronavirus
Yellow fever virus

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2010.10.008

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@article{0c65009e1484416a93ed4cc59eb25c4a,

title = "Antiviral activities of ISG20 in positive-strand RNA virus infections",

abstract = "ISG20 is an interferon-inducible 3-5exonuclease that inhibits replication of several human and animal RNA viruses. However, the specificities of ISG20's antiviral action remain poorly defined. Here we determine the impact of ectopic expression of ISG20 on replication of several positive-strand RNA viruses from distinct viral families. ISG20 inhibited infections by cell culture-derived hepatitis C virus (HCV) and a pestivirus, bovine viral diarrhea virus and a picornavirus, hepatitis A virus. Moreover, ISG20 demonstrated cell-type specific antiviral activity against yellow fever virus, a classical flavivirus. Overexpression of ISG20, however, did not inhibit propagation of severe acute respiratory syndrome coronavirus, a highly-pathogenic human coronavirus in Huh7.5 cells. The antiviral effects of ISG20 were all dependent on its exonuclease activity. The closely related cellular exonucleases, ISG20L1 and ISG20L2, did not inhibit HCV replication. Together, these data may help better understand the antiviral specificity and action of ISG20.",

keywords = "Antiviral, Bovine viral diarrhea virus, Hepatitis A virus, Hepatitis C virus, ISG20, ISG20L1, ISG20L2, Innate immunity, Interferon, Severe acute respiratory syndrome coronavirus, Yellow fever virus",

author = "Zhi Zhou and Nan Wang and Woodson, {Sara E.} and Qingming Dong and Jie Wang and Yuqiong Liang and Rene Rijnbrand and Lai Wei and Nichols, {Joan E.} and Guo, {Ju Tao} and Holbrook, {Michael R.} and Lemon, {Stanley M.} and Kui Li",

note = "Funding Information: This work was supported in part by NIH grants R01-AI069285 (KL) and U19-AI040035 and R21-AI081058 (SML) and an American Cancer Society Institutional Research Grant 96-152-07 (KL). ZZ was supported by a Visiting Scholarship from the China Scholarship Council and a Natural Science Foundation Project of Chongqing ( CSTC, 2009BB5063 ). We thank Takaji Wakita for JFH1 cDNAs, Charles Rice for Huh7.5 cells, YFV NS3 antiserum and pFL-J6/JFH-5′C19Rluc2AUbi cDNA, John McLauchlan for HCV core antiserum, Guangxiang Luo for pSGR-JFH1-Blast cDNA, Robert Tesh and Alan Barrett for YFVs, Thomas Ksiazek and Lia Haynes for SARS-CoV. ",

year = "2011",

month = jan,

day = "20",

doi = "10.1016/j.virol.2010.10.008",

language = "English (US)",

volume = "409",

pages = "175--188",

journal = "Virology",

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publisher = "Academic Press",

number = "2",

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T1 - Antiviral activities of ISG20 in positive-strand RNA virus infections

AU - Zhou, Zhi

AU - Wang, Nan

AU - Woodson, Sara E.

AU - Dong, Qingming

AU - Wang, Jie

AU - Liang, Yuqiong

AU - Rijnbrand, Rene

AU - Wei, Lai

AU - Nichols, Joan E.

AU - Guo, Ju Tao

AU - Holbrook, Michael R.

AU - Lemon, Stanley M.

AU - Li, Kui

N1 - Funding Information: This work was supported in part by NIH grants R01-AI069285 (KL) and U19-AI040035 and R21-AI081058 (SML) and an American Cancer Society Institutional Research Grant 96-152-07 (KL). ZZ was supported by a Visiting Scholarship from the China Scholarship Council and a Natural Science Foundation Project of Chongqing ( CSTC, 2009BB5063 ). We thank Takaji Wakita for JFH1 cDNAs, Charles Rice for Huh7.5 cells, YFV NS3 antiserum and pFL-J6/JFH-5′C19Rluc2AUbi cDNA, John McLauchlan for HCV core antiserum, Guangxiang Luo for pSGR-JFH1-Blast cDNA, Robert Tesh and Alan Barrett for YFVs, Thomas Ksiazek and Lia Haynes for SARS-CoV.

PY - 2011/1/20

Y1 - 2011/1/20

N2 - ISG20 is an interferon-inducible 3-5exonuclease that inhibits replication of several human and animal RNA viruses. However, the specificities of ISG20's antiviral action remain poorly defined. Here we determine the impact of ectopic expression of ISG20 on replication of several positive-strand RNA viruses from distinct viral families. ISG20 inhibited infections by cell culture-derived hepatitis C virus (HCV) and a pestivirus, bovine viral diarrhea virus and a picornavirus, hepatitis A virus. Moreover, ISG20 demonstrated cell-type specific antiviral activity against yellow fever virus, a classical flavivirus. Overexpression of ISG20, however, did not inhibit propagation of severe acute respiratory syndrome coronavirus, a highly-pathogenic human coronavirus in Huh7.5 cells. The antiviral effects of ISG20 were all dependent on its exonuclease activity. The closely related cellular exonucleases, ISG20L1 and ISG20L2, did not inhibit HCV replication. Together, these data may help better understand the antiviral specificity and action of ISG20.

AB - ISG20 is an interferon-inducible 3-5exonuclease that inhibits replication of several human and animal RNA viruses. However, the specificities of ISG20's antiviral action remain poorly defined. Here we determine the impact of ectopic expression of ISG20 on replication of several positive-strand RNA viruses from distinct viral families. ISG20 inhibited infections by cell culture-derived hepatitis C virus (HCV) and a pestivirus, bovine viral diarrhea virus and a picornavirus, hepatitis A virus. Moreover, ISG20 demonstrated cell-type specific antiviral activity against yellow fever virus, a classical flavivirus. Overexpression of ISG20, however, did not inhibit propagation of severe acute respiratory syndrome coronavirus, a highly-pathogenic human coronavirus in Huh7.5 cells. The antiviral effects of ISG20 were all dependent on its exonuclease activity. The closely related cellular exonucleases, ISG20L1 and ISG20L2, did not inhibit HCV replication. Together, these data may help better understand the antiviral specificity and action of ISG20.

KW - Antiviral

KW - Bovine viral diarrhea virus

KW - Hepatitis A virus

KW - Hepatitis C virus

KW - ISG20

KW - ISG20L1

KW - ISG20L2

KW - Innate immunity

KW - Interferon

KW - Severe acute respiratory syndrome coronavirus

KW - Yellow fever virus

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U2 - 10.1016/j.virol.2010.10.008

DO - 10.1016/j.virol.2010.10.008

M3 - Article

C2 - 21036379

AN - SCOPUS:78650295825

SN - 0042-6822

VL - 409

SP - 175

EP - 188

JO - Virology

JF - Virology

IS - 2

ER -

Antiviral activities of ISG20 in positive-strand RNA virus infections

Abstract

Keywords

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