Application of Laser Self-Beat Spectroscopic Technique to the Study of Solutions of Human Plasma Low-Density Lipoproteins

R. W. DeBlois; E. E. Uzgiris; S. K. Devi; Antonio Gotto

doi:10.1021/bi00738a015

Application of Laser Self-Beat Spectroscopic Technique to the Study of Solutions of Human Plasma Low-Density Lipoproteins

R. W. DeBlois, E. E. Uzgiris, S. K. Devi, Antonio Gotto

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16 Scopus citations

Abstract

The technique of self-beat or homodyne spectroscopy has been applied to the determination of the translational diffusion constant of human plasma low-density lipoproteins (LDL). Both power spectrum and autocorrelation methods give equivalent results, but with conventional equipment the power spectrum measurement takes on the order of hours while autocorrelation is accomplished in minutes. The effects of concentration of LDL, scattering angle of the light pH of the solution and buffer concentration were investigated. The diffusion constant obtained, D_25,w = 2.14 ± 0.09 × 10^-7 cm²/sec, was in good general accord with conventional measures. The equivalent spherical diameter, obtained from the Stokes-Einstein relationship, 229 ± 10 Å was within a broad band of values given by other techniques.

Original language	English (US)
Pages (from-to)	2645-2649
Number of pages	5
Journal	Biochemistry
Volume	12
Issue number	14
DOIs	https://doi.org/10.1021/bi00738a015
State	Published - Jul 1 1973

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Biochemistry

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title = "Application of Laser Self-Beat Spectroscopic Technique to the Study of Solutions of Human Plasma Low-Density Lipoproteins",

abstract = "The technique of self-beat or homodyne spectroscopy has been applied to the determination of the translational diffusion constant of human plasma low-density lipoproteins (LDL). Both power spectrum and autocorrelation methods give equivalent results, but with conventional equipment the power spectrum measurement takes on the order of hours while autocorrelation is accomplished in minutes. The effects of concentration of LDL, scattering angle of the light pH of the solution and buffer concentration were investigated. The diffusion constant obtained, D25,w = 2.14 ± 0.09 × 10-7 cm2/sec, was in good general accord with conventional measures. The equivalent spherical diameter, obtained from the Stokes-Einstein relationship, 229 ± 10 {\AA} was within a broad band of values given by other techniques.",

author = "DeBlois, {R. W.} and Uzgiris, {E. E.} and Devi, {S. K.} and Antonio Gotto",

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T1 - Application of Laser Self-Beat Spectroscopic Technique to the Study of Solutions of Human Plasma Low-Density Lipoproteins

AU - DeBlois, R. W.

AU - Uzgiris, E. E.

AU - Devi, S. K.

AU - Gotto, Antonio

PY - 1973/7/1

Y1 - 1973/7/1

N2 - The technique of self-beat or homodyne spectroscopy has been applied to the determination of the translational diffusion constant of human plasma low-density lipoproteins (LDL). Both power spectrum and autocorrelation methods give equivalent results, but with conventional equipment the power spectrum measurement takes on the order of hours while autocorrelation is accomplished in minutes. The effects of concentration of LDL, scattering angle of the light pH of the solution and buffer concentration were investigated. The diffusion constant obtained, D25,w = 2.14 ± 0.09 × 10-7 cm2/sec, was in good general accord with conventional measures. The equivalent spherical diameter, obtained from the Stokes-Einstein relationship, 229 ± 10 Å was within a broad band of values given by other techniques.

AB - The technique of self-beat or homodyne spectroscopy has been applied to the determination of the translational diffusion constant of human plasma low-density lipoproteins (LDL). Both power spectrum and autocorrelation methods give equivalent results, but with conventional equipment the power spectrum measurement takes on the order of hours while autocorrelation is accomplished in minutes. The effects of concentration of LDL, scattering angle of the light pH of the solution and buffer concentration were investigated. The diffusion constant obtained, D25,w = 2.14 ± 0.09 × 10-7 cm2/sec, was in good general accord with conventional measures. The equivalent spherical diameter, obtained from the Stokes-Einstein relationship, 229 ± 10 Å was within a broad band of values given by other techniques.

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Application of Laser Self-Beat Spectroscopic Technique to the Study of Solutions of Human Plasma Low-Density Lipoproteins

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