Abstract
Mycoplasma contamination of cell line cultures is a common, yet often undetected problem in research laboratories. Many of the existing techniques to detect mycoplasma contamination of cultured cells are time-consuming, expensive, and have significant drawbacks. Here, we describe a mycoplasma detection system that is useful for detecting multiple species of mycoplasma in infected cell lines. The system contains three dye-labeled detection aptamers that can specifically bind to mycoplasma-infected cells and a dye-labeled control aptamer that minimally binds to cells. With this system, mycoplasma-contaminated cells can be detected within 30 min by using a flow cytometer, fluorescence microscope, or microplate reader. Further, this system may be used to detect mycoplasma-contaminated culture medium. This study presents an novel mycoplasma detection model that is simple, rapid, inexpensive, and sensitive.
Original language | English (US) |
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Article number | 3784 |
Journal | International journal of molecular sciences |
Volume | 21 |
Issue number | 11 |
DOIs | |
State | Published - May 27 2020 |
Keywords
- Cell culture
- DNA aptamer
- Mycoplasma
- Rapid detection
- Binding, Competitive
- Humans
- Flow Cytometry
- Culture Media
- DNA Contamination
- Cell Line, Tumor
- Cell Culture Techniques
- Mycoplasma/genetics
- Aptamers, Nucleotide/metabolism
ASJC Scopus subject areas
- Molecular Biology
- Spectroscopy
- Catalysis
- Inorganic Chemistry
- Computer Science Applications
- Physical and Theoretical Chemistry
- Organic Chemistry