@article{c2c6a6dcec77466ca3ab1746a4b09e48,
title = "Bacteroides ovatus colonization influences the abundance of intestinal short chain fatty acids and neurotransmitters",
abstract = "Gut microbes can synthesize multiple neuro-active metabolites. We profiled neuro-active compounds produced by the gut commensal Bacteroides ovatus in vitro and in vivo by LC-MS/MS. We found that B. ovatus generates acetic acid, propionic acid, isobutyric acid, and isovaleric acid. In vitro, B. ovatus consumed tryptophan and glutamate and synthesized the neuro-active compounds glutamine and GABA. Consistent with our LC-MS/MS-based in vitro data, we observed elevated levels of acetic acid, propionic acid, isobutyric acid, and isovaleric acid in the intestines of B. ovatus mono-associated mice compared with germ-free controls. B. ovatus mono-association also increased the concentrations of intestinal GABA and decreased the concentrations of tryptophan and glutamine compared with germ-free controls. Computational network analysis revealed unique links between SCFAs, neuro-active compounds, and colonization status. These results highlight connections between microbial colonization and intestinal neurotransmitter concentrations, suggesting that B. ovatus selectively influences the presence of intestinal neurotransmitters.",
keywords = "Microbiology, Microbiome, Neuroscience",
author = "Horvath, {Thomas D.} and Ihekweazu, {Faith D.} and Haidacher, {Sigmund J.} and Wenly Ruan and Engevik, {Kristen A.} and Robert Fultz and Hoch, {Kathleen M.} and Luna, {Ruth Ann} and Numan Oezguen and Spinler, {Jennifer K.} and Haag, {Anthony M.} and James Versalovic and Engevik, {Melinda A.}",
note = "Funding Information: The Texas Children's Hospital Department of Pathology and Immunology provides salary support to Texas Children's Microbiome Center-Metabolomics Lab staff, and purchased all of the reagents, the consumables and durable supplies, and the LC-MS/MS equipment described. Concept and design (T.D.H. F.D.A. A.M.H. J.V. M.A.E.); intellectual contribution (T.D.H. F.D.I. S.J.H. K.A.E. R.F. K.M.H. N.O. J.K.S. A.M.H. J.V. M.A.E.); data acquisition (T.D.H. F.D.I. S.J.H. K.A.E. R.F. K.M.H. M.A.E.); data analysis, statistics, and interpretation (T.D.H. F.D.I. S.J.H. K.M.H. N.O. J.K.S. A.M.H. M.A.E.); editing manuscript (T.D.H. F.D.I. S.J.H. K.A.E. R.F. K.M.H. N.O. J.K.S. A.M.H. J.V. M.A.E.); funding (F.D.I. A.M.H. J.V. M.A.E.). J.V. serves on the scientific advisory board of Seed, a USA-based probiotics/prebiotics company, Biomica, an Israeli informatics enterprise. and Plexus World-wide, a USA-based nutrition company. J.S.K. and J.V. receive unrestricted research support from BioGaia AB, a Swedish probiotics company. The remaining authors have no commercial or financial relationships to declare. Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2022",
month = may,
day = "20",
doi = "10.1016/j.isci.2022.104158",
language = "English (US)",
volume = "25",
pages = "104158",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier",
number = "5",
}