Base Pairing and Template Specificity During Deoxyribonucleic Acid Repair Synthesis in Human and Mouse Cells

Michael W. Lieberman; Miriam C. Poirier

doi:10.1021/bi00723a021

Base Pairing and Template Specificity During Deoxyribonucleic Acid Repair Synthesis in Human and Mouse Cells

Michael W. Lieberman, Miriam C. Poirier

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Nuclcotide insertion during DNA repair synthesis has been studied in cultured human diploid fibroblasts (WI-38) and Balb/c 3T3 mouse cells damaged with ultraviolet radiation and two mutagenic carcinogens, N-acetoxy-2-acetylaminofluorene (NA-AAF) and 7-bromomethylbenz[a]anthracene (7BrMeBA). Evidence from thermal elution chromatography, S₁ nuclease digestion studies, and pyrimidine isostich analysis suggests that in mammalian cells the complementary, undamaged strand serves as a template for repair synthesis and that the result of such synthesis is the accurate restoration of the damaged area to the original nucleotide sequence.

Original language	English (US)
Pages (from-to)	5384-5388
Number of pages	5
Journal	Biochemistry
Volume	13
Issue number	26
DOIs	https://doi.org/10.1021/bi00723a021
State	Published - Dec 1 1974

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Biochemistry

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title = "Base Pairing and Template Specificity During Deoxyribonucleic Acid Repair Synthesis in Human and Mouse Cells",

abstract = "Nuclcotide insertion during DNA repair synthesis has been studied in cultured human diploid fibroblasts (WI-38) and Balb/c 3T3 mouse cells damaged with ultraviolet radiation and two mutagenic carcinogens, N-acetoxy-2-acetylaminofluorene (NA-AAF) and 7-bromomethylbenz[a]anthracene (7BrMeBA). Evidence from thermal elution chromatography, S1 nuclease digestion studies, and pyrimidine isostich analysis suggests that in mammalian cells the complementary, undamaged strand serves as a template for repair synthesis and that the result of such synthesis is the accurate restoration of the damaged area to the original nucleotide sequence.",

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year = "1974",

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doi = "10.1021/bi00723a021",

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T1 - Base Pairing and Template Specificity During Deoxyribonucleic Acid Repair Synthesis in Human and Mouse Cells

AU - Lieberman, Michael W.

AU - Poirier, Miriam C.

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Y1 - 1974/12/1

N2 - Nuclcotide insertion during DNA repair synthesis has been studied in cultured human diploid fibroblasts (WI-38) and Balb/c 3T3 mouse cells damaged with ultraviolet radiation and two mutagenic carcinogens, N-acetoxy-2-acetylaminofluorene (NA-AAF) and 7-bromomethylbenz[a]anthracene (7BrMeBA). Evidence from thermal elution chromatography, S1 nuclease digestion studies, and pyrimidine isostich analysis suggests that in mammalian cells the complementary, undamaged strand serves as a template for repair synthesis and that the result of such synthesis is the accurate restoration of the damaged area to the original nucleotide sequence.

AB - Nuclcotide insertion during DNA repair synthesis has been studied in cultured human diploid fibroblasts (WI-38) and Balb/c 3T3 mouse cells damaged with ultraviolet radiation and two mutagenic carcinogens, N-acetoxy-2-acetylaminofluorene (NA-AAF) and 7-bromomethylbenz[a]anthracene (7BrMeBA). Evidence from thermal elution chromatography, S1 nuclease digestion studies, and pyrimidine isostich analysis suggests that in mammalian cells the complementary, undamaged strand serves as a template for repair synthesis and that the result of such synthesis is the accurate restoration of the damaged area to the original nucleotide sequence.

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JO - Biochemistry

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Base Pairing and Template Specificity During Deoxyribonucleic Acid Repair Synthesis in Human and Mouse Cells

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