Abstract
We report the release of catalytic DNA walkers from hydrogel microparticles and the detection of those walkers by substrate-coated microparticles. This might be considered a synthetic biology analog of molecular signal release and reception. One type of particles was coated with components of a DNA one-step strand displacement (OSD) reaction to release the walker. A second type of particle was coated with substrate (or "track") for the molecular walker. We distinguish these particle types using fluorescence barcoding: we synthesized and distinguished multiple particle types with multicolor fluorescence microscopy and automated image analysis software. This represents a step toward amplified, multiplex, and microscopically localized detection based on DNA nanotechnology.
Original language | English (US) |
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Pages (from-to) | 4081 |
Journal | Scientific Reports |
Volume | 7 |
Issue number | 1 |
DOIs | |
State | Published - Jun 22 2017 |
Keywords
- Biomimetics/methods
- DNA/chemistry
- DNA, Catalytic
- Fluorescent Dyes
- Hydrogels/chemistry
- Nanoparticles/chemistry
- Nanotechnology