TY - JOUR
T1 - Biomimetic small exosome with outstanding surgical applications for rapid large-scale wound healing and functional sweat gland restoration
AU - Chen, Huating
AU - Liu, Yawei
AU - Liu, Yiqiong
AU - Ji, Shuaifei
AU - Xiang, Jiangbing
AU - Li, Yan
AU - Zhou, Laixian
AU - Gao, Huanhuan
AU - Deng, Zihui
AU - Li, Binghui
AU - Sun, Sujing
AU - Cui, Shaoyuan
AU - Li, Gongchi
AU - Sheng, Wei
AU - Liu, Huiling
AU - Chen, Chunying
AU - Zhao, Yuliang
AU - Zhang, Hongjie
AU - Liu, Kai
AU - Fu, Xiaobing
AU - Sun, Xiaoyan
N1 - Funding Information:
H.C and Y.L. contributed equally to this work. This work is supported in part by National Natural Science Foundation of China ( 81871569 , 81830064 , 81721092 , 61803250 , 22125701 , 22020102003 ), the National Key Research and Development Plan ( 2018YFC1105704 , 2020YFA0908900 , 2017YFC1103304 , 2016YFA0101000 , 2016YFA0101002 ), the CAMS Innovation Fund for Medical Sciences (CIFMS, 2019-I2M-5-059 ), Tsinghua University Spring Breeze Fund ( 2021Z99CFZ005 ), the Military Key Basic Research of Foundational Strengthening Program ( 2020-JCJQ-ZD-256-021 ), Military Medical Research and Development Projects ( AWS17J005 , 2019-126 ) and China Postdoctoral Science Foundation ( 2020M681055 ).
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/8
Y1 - 2022/8
N2 - The realization of coordinated infiltration of dermal and epidermal cell types, and the rapid deposition of extracellular matrix are the prerequisites for improved healing quality of large skin defects. However, presently used materials are limited for wound healing due to the inherent poor histocompatibility, low permeability, poor mechanical property and cytotoxicity. It is challenging to develop new therapeutic agents that enable timely and efficient wound healing and meanwhile there are minimal side effects to avoid chronic wounds, wound adhesions, and large scarring. Herein, we fabricated biomimetic exosomes (EMs) that enriched transforming growth factor β1 (TGF-β1) for the rapid healing of large-scale cutaneous wounds. The EM encapsulation allowed well-managed dosing of the TGF-β1 to effectively promote the wound healing process by endowing epidermal keratinocytes with a migratory feature, enhancing their stem cell properties, and resulting in accelerated in vivo wound re-epithelization. Specifically, the EMs could fast restore the function of sweat glands in a thermally injured mice model. Our work provides a promising strategy for improving the healing speed and healing quality for patients with large wounds.
AB - The realization of coordinated infiltration of dermal and epidermal cell types, and the rapid deposition of extracellular matrix are the prerequisites for improved healing quality of large skin defects. However, presently used materials are limited for wound healing due to the inherent poor histocompatibility, low permeability, poor mechanical property and cytotoxicity. It is challenging to develop new therapeutic agents that enable timely and efficient wound healing and meanwhile there are minimal side effects to avoid chronic wounds, wound adhesions, and large scarring. Herein, we fabricated biomimetic exosomes (EMs) that enriched transforming growth factor β1 (TGF-β1) for the rapid healing of large-scale cutaneous wounds. The EM encapsulation allowed well-managed dosing of the TGF-β1 to effectively promote the wound healing process by endowing epidermal keratinocytes with a migratory feature, enhancing their stem cell properties, and resulting in accelerated in vivo wound re-epithelization. Specifically, the EMs could fast restore the function of sweat glands in a thermally injured mice model. Our work provides a promising strategy for improving the healing speed and healing quality for patients with large wounds.
KW - Biomimetic materials
KW - Exosome
KW - Skin regeneration
KW - Sweat glands
KW - Wound healing
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U2 - 10.1016/j.nantod.2022.101531
DO - 10.1016/j.nantod.2022.101531
M3 - Article
AN - SCOPUS:85132888128
SN - 1748-0132
VL - 45
JO - Nano Today
JF - Nano Today
M1 - 101531
ER -