TY - JOUR
T1 - Carcinogenic Helicobacter pylori in gastric pre-cancer and cancer lesions
T2 - Association with tobacco-chewing
AU - Pandey, Arvind
AU - Tripathi, Satyendra Chandra
AU - Mahata, Sutapa
AU - Vishnoi, Kanchan
AU - Shukla, Shirish
AU - Misra, Sri Prakash
AU - Misra, Vatsala
AU - Hedau, Suresh
AU - Mehrotra, Ravi
AU - Dwivedi, Manisha
AU - Bharti, Alok C.
N1 - Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2014
Y1 - 2014
N2 - Aim: To investigate the low gastric cancer incidence rate relative to the highly prevalent Helicobacter pylori (H. pylori) infection; data relevant to H. pylori infection during gastric carcinogenesis in Indian patients is currently lacking. Methods: The present study examines the prevalence of H. pylori infection in DNA derived from 156 endoscopic gastric biopsies of different disease groups that represent gastric pre-cancer [intestinal metaplasia (n = 15), dysplasia (n = 15)], cancer [diffuse adenocarcinoma (n = 44), intestinal adenocarcinoma (n = 21)], and symptomatic but histopathologically-normal controls (n = 61). This was done by generic ureC polymerase chain reaction (PCR) and cagA -specific PCR that could specifically identify the carcinogenic H. pylori strain. Results: Our analysis showed the presence of H. pylori infection in 61% of symptomatic histopathologically- normal individuals, however only 34% of control tissues were harboring the cagA+ H. pylori strain. A similar proportion of H. pylori infection (52%) and cagA (26%) positivity was observed in the tumor tissue of the gastric cancer group. In comparison, H. pylori infection (90%) and cagA positivity (73%) were the highest in gastric pre-cancer lesions. In relation to tobacco and alcohol abuse, H. pylori infection showed an association with tobacco chewing, whereas we did not observe any association between tobacco smoking or alcohol abuse with prevalence of H. pylori infection in the tissue of any of the patient groups studied. Conclusion: High incidence of H. pylori infection and carcinogenic cagA positive strain in pre-cancer lesions during gastric carcinogenesis may be associated
AB - Aim: To investigate the low gastric cancer incidence rate relative to the highly prevalent Helicobacter pylori (H. pylori) infection; data relevant to H. pylori infection during gastric carcinogenesis in Indian patients is currently lacking. Methods: The present study examines the prevalence of H. pylori infection in DNA derived from 156 endoscopic gastric biopsies of different disease groups that represent gastric pre-cancer [intestinal metaplasia (n = 15), dysplasia (n = 15)], cancer [diffuse adenocarcinoma (n = 44), intestinal adenocarcinoma (n = 21)], and symptomatic but histopathologically-normal controls (n = 61). This was done by generic ureC polymerase chain reaction (PCR) and cagA -specific PCR that could specifically identify the carcinogenic H. pylori strain. Results: Our analysis showed the presence of H. pylori infection in 61% of symptomatic histopathologically- normal individuals, however only 34% of control tissues were harboring the cagA+ H. pylori strain. A similar proportion of H. pylori infection (52%) and cagA (26%) positivity was observed in the tumor tissue of the gastric cancer group. In comparison, H. pylori infection (90%) and cagA positivity (73%) were the highest in gastric pre-cancer lesions. In relation to tobacco and alcohol abuse, H. pylori infection showed an association with tobacco chewing, whereas we did not observe any association between tobacco smoking or alcohol abuse with prevalence of H. pylori infection in the tissue of any of the patient groups studied. Conclusion: High incidence of H. pylori infection and carcinogenic cagA positive strain in pre-cancer lesions during gastric carcinogenesis may be associated
KW - CagA
KW - Dysplasia
KW - Gastric adenocarcinoma
KW - Intestinal metaplasia
KW - Tobacco chewing
KW - UreC
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U2 - 10.3748/wjg.v20.i22.6860
DO - 10.3748/wjg.v20.i22.6860
M3 - Article
C2 - 24944476
AN - SCOPUS:84902197769
SN - 1007-9327
VL - 20
SP - 6860
EP - 6868
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
IS - 22
ER -