Cellular reagents for diagnostics and synthetic biology

Sanchita Bhadra; Arti Pothukuchy; Raghav Shroff; Austin W. Cole; Michelle Byrom; Jared W. Ellefson; Jimmy D. Gollihar; Andrew D. Ellington

doi:10.1371/journal.pone.0201681

Cellular reagents for diagnostics and synthetic biology

Sanchita Bhadra, Arti Pothukuchy, Raghav Shroff, Austin W. Cole, Michelle Byrom, Jared W. Ellefson, Jimmy D. Gollihar, Andrew D. Ellington

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13 Scopus citations

Abstract

We have found that the overproduction of enzymes in bacteria followed by their lyophilization leads to 'cellular reagents' that can be directly used to carry out numerous molecular biology reactions. We demonstrate the use of cellular reagents in a variety of molecular diagnostics, such as TaqMan qPCR with no diminution in sensitivity, and in synthetic biology cornerstones such as the Gibson assembly of DNA fragments, where new plasmids can be constructed solely based on adding cellular reagents. Cellular reagents have significantly reduced complexity and cost of production, storage and implementation, features that should facilitate accessibility and use in resource-poor conditions.

Original language	English (US)
Article number	e0201681
Journal	PLoS ONE
Volume	13
Issue number	8
DOIs	https://doi.org/10.1371/journal.pone.0201681
State	Published - Aug 2018

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title = "Cellular reagents for diagnostics and synthetic biology",

abstract = "We have found that the overproduction of enzymes in bacteria followed by their lyophilization leads to 'cellular reagents' that can be directly used to carry out numerous molecular biology reactions. We demonstrate the use of cellular reagents in a variety of molecular diagnostics, such as TaqMan qPCR with no diminution in sensitivity, and in synthetic biology cornerstones such as the Gibson assembly of DNA fragments, where new plasmids can be constructed solely based on adding cellular reagents. Cellular reagents have significantly reduced complexity and cost of production, storage and implementation, features that should facilitate accessibility and use in resource-poor conditions.",

author = "Sanchita Bhadra and Arti Pothukuchy and Raghav Shroff and Cole, {Austin W.} and Michelle Byrom and Ellefson, {Jared W.} and Gollihar, {Jimmy D.} and Ellington, {Andrew D.}",

note = "Publisher Copyright: Copyright {\textcopyright} 2018 Bhadra et al.",

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AU - Bhadra, Sanchita

AU - Pothukuchy, Arti

AU - Shroff, Raghav

AU - Cole, Austin W.

AU - Byrom, Michelle

AU - Ellefson, Jared W.

AU - Gollihar, Jimmy D.

AU - Ellington, Andrew D.

PY - 2018/8

Y1 - 2018/8

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AB - We have found that the overproduction of enzymes in bacteria followed by their lyophilization leads to 'cellular reagents' that can be directly used to carry out numerous molecular biology reactions. We demonstrate the use of cellular reagents in a variety of molecular diagnostics, such as TaqMan qPCR with no diminution in sensitivity, and in synthetic biology cornerstones such as the Gibson assembly of DNA fragments, where new plasmids can be constructed solely based on adding cellular reagents. Cellular reagents have significantly reduced complexity and cost of production, storage and implementation, features that should facilitate accessibility and use in resource-poor conditions.

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JO - PLoS ONE

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Cellular reagents for diagnostics and synthetic biology

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