TY - JOUR
T1 - Characterization of the subcellular localization of herpes simplex virus type 1 proteins in living cells
AU - Xing, Junji
AU - Wang, Shuai
AU - Li, You
AU - Guo, Hong
AU - Zhao, Lei
AU - Pan, Weiwei
AU - Lin, Fusen
AU - Zhu, Huifang
AU - Wang, Lei
AU - Li, Meili
AU - Wang, Lin
AU - Zheng, Chunfu
N1 - Funding Information:
Acknowledgments This study was supported by the Major State Basic Research Development Program of China (2010CB530105 and 2011CB504802); the National Natural Science Foundation of China (30900059, 30870120 and 81000736); the Start-up Fund of the Hundred Talents Program of the Chinese Academy of Sciences (20071010-141).
Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2011/2
Y1 - 2011/2
N2 - In this study, we presented the construction of a library of expression clones for the herpes simplex virus type 1 (HSV-1) proteome and subcellular localization map of HSV-1 proteins in living cells using yellow fluorescent protein (YFP) fusion proteins. As a result, 21 proteins showed cytoplasmic or subcytoplasmic localization, 16 proteins showed nuclear or subnuclear localization, and others were present both in the nucleus and cytoplasm. Interestingly, most capsid proteins showed enriched or exclusive localization in the nucleus, and most of the envelope proteins showed cytoplasmic localization, suggesting that subcellular localization of the proteins correlated with their functions during virus replication. These results present a subcellular localization map of HSV-1 proteins in living cells, which provide useful information to further characterize the functions of these proteins.
AB - In this study, we presented the construction of a library of expression clones for the herpes simplex virus type 1 (HSV-1) proteome and subcellular localization map of HSV-1 proteins in living cells using yellow fluorescent protein (YFP) fusion proteins. As a result, 21 proteins showed cytoplasmic or subcytoplasmic localization, 16 proteins showed nuclear or subnuclear localization, and others were present both in the nucleus and cytoplasm. Interestingly, most capsid proteins showed enriched or exclusive localization in the nucleus, and most of the envelope proteins showed cytoplasmic localization, suggesting that subcellular localization of the proteins correlated with their functions during virus replication. These results present a subcellular localization map of HSV-1 proteins in living cells, which provide useful information to further characterize the functions of these proteins.
KW - Herpes simplex virus type 1 proteins
KW - Living cell microscopy
KW - Subcellular localization
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U2 - 10.1007/s00430-010-0175-9
DO - 10.1007/s00430-010-0175-9
M3 - Article
C2 - 20949280
AN - SCOPUS:78751629252
SN - 0300-8584
VL - 200
SP - 61
EP - 68
JO - Medical Microbiology and Immunology
JF - Medical Microbiology and Immunology
IS - 1
ER -