Abstract
We have isolated and characterized a complete structural gene encoding the enzyme γ-glutamyltransferase ((5-glutamyl)-peptide:amino acid 5-glutamyltransferase; EC 2.3.2.2). The gene contains 8 exons and spans ∼12 kilobases. Ras-transformed rat liver epithelial cells and rat kidney express RNAs which differ in length by ∼0.3 kilobase pair. Comparison of the genomic sequence with kidney γGT cDNA sequence indicates that the first exon is noncoding, and nuclease protection and primer extension data have identified a potential kidney transcription start site (defined as +1) for this exon. The site is not associated with a TATA box, but there are two CCAAT boxes (-136 and -599) and two sites (-101 and -746) containing the consensus sequences to which the transcription factor SP1 is known to bind. There is also a sequence at -453 (TGTGGTTG) that is highly homologous to the core sequence (TGTGG(T)3-5G) of SV40 and polyoma viral enhancers.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 11721-11725 |
| Number of pages | 5 |
| Journal | Journal of Biological Chemistry |
| Volume | 265 |
| Issue number | 20 |
| State | Published - 1990 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology