Coinjection strategy for visual identification of transgenic mice

Paul A. Overbeek; Estuardo Aguilar-Cordova; Gerri Hanten; David L. Schaffner; Parul Patel; Russell M. Lebovitz; Michael W. Lieberman

doi:10.1007/BF02512994

Coinjection strategy for visual identification of transgenic mice

Paul A. Overbeek, Estuardo Aguilar-Cordova, Gerri Hanten, David L. Schaffner, Parul Patel, Russell M. Lebovitz, Michael W. Lieberman

Research output: Contribution to journal › Article › peer-review

77 Scopus citations

Abstract

Transgenic mice were generated by coinjection of a dominant marker gene that induces fur and eye pigmentation (a tyrosinase minigene) plus an unrelated DNA construction that has a γ-glutamyl transferase (γGT) promoter linked to a ras oncogene. Mice transgenic for γGT-ras could be identified in the first and all subsequent generations by simple visual inspection for pigmentation. Furthermore, the γ-glutamyl transferase promoter was active in kidney but not skin of the transgenic mice, indicating that the cointegrated DNA was active and independently expressed. These results confirm that the tyrosinase minigene can be used for coinjections to allow rapid visual identification of transgenic mice.

Original language	English (US)
Pages (from-to)	31-37
Number of pages	7
Journal	Transgenic Research
Volume	1
Issue number	1
DOIs	https://doi.org/10.1007/BF02512994
State	Published - Dec 1991

Keywords

γ-glutamyl transferase
cointegration
ras T24 oncogene
tyrosinase

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Molecular Biology
Genetics
Biotechnology
Plant Science
Food Science

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10.1007/BF02512994

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title = "Coinjection strategy for visual identification of transgenic mice",

abstract = "Transgenic mice were generated by coinjection of a dominant marker gene that induces fur and eye pigmentation (a tyrosinase minigene) plus an unrelated DNA construction that has a γ-glutamyl transferase (γGT) promoter linked to a ras oncogene. Mice transgenic for γGT-ras could be identified in the first and all subsequent generations by simple visual inspection for pigmentation. Furthermore, the γ-glutamyl transferase promoter was active in kidney but not skin of the transgenic mice, indicating that the cointegrated DNA was active and independently expressed. These results confirm that the tyrosinase minigene can be used for coinjections to allow rapid visual identification of transgenic mice.",

keywords = "γ-glutamyl transferase, cointegration, ras T24 oncogene, tyrosinase",

author = "Overbeek, {Paul A.} and Estuardo Aguilar-Cordova and Gerri Hanten and Schaffner, {David L.} and Parul Patel and Lebovitz, {Russell M.} and Lieberman, {Michael W.}",

year = "1991",

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T1 - Coinjection strategy for visual identification of transgenic mice

AU - Overbeek, Paul A.

AU - Aguilar-Cordova, Estuardo

AU - Hanten, Gerri

AU - Schaffner, David L.

AU - Patel, Parul

AU - Lebovitz, Russell M.

AU - Lieberman, Michael W.

PY - 1991/12

Y1 - 1991/12

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AB - Transgenic mice were generated by coinjection of a dominant marker gene that induces fur and eye pigmentation (a tyrosinase minigene) plus an unrelated DNA construction that has a γ-glutamyl transferase (γGT) promoter linked to a ras oncogene. Mice transgenic for γGT-ras could be identified in the first and all subsequent generations by simple visual inspection for pigmentation. Furthermore, the γ-glutamyl transferase promoter was active in kidney but not skin of the transgenic mice, indicating that the cointegrated DNA was active and independently expressed. These results confirm that the tyrosinase minigene can be used for coinjections to allow rapid visual identification of transgenic mice.

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KW - cointegration

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Coinjection strategy for visual identification of transgenic mice

Abstract

Keywords

ASJC Scopus subject areas

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