TY - JOUR
T1 - Combined Effect of Citrate and Fluoride Ions on Hydroxyapatite Nanoparticles
AU - Degli Esposti, Lorenzo
AU - Adamiano, Alessio
AU - Tampieri, Anna
AU - Ramirez-Rodriguez, Gloria Belen
AU - Siliqi, Dritan
AU - Giannini, Cinzia
AU - Ivanchenko, Pavlo
AU - Martra, Gianmario
AU - Lin, Feng Huei
AU - Delgado-López, José Manuel
AU - Iafisco, Michele
N1 - Funding Information:
The authors acknowledge the CERIC-ERIC Consortium for the access to experimental facilities and financial support under Proposal CERIC-ERIC-20175403, and Dr. Heinz Amenitsch of Austrian SAXS beamline of the ELETTRA Synchrotron for the scientific and technical support provided during data collection experiments. J.M.D.-L and G.B.R.-R. acknowledge the Ministry of Science, Innovation and Universities of Spain (MCIU/AEI/FEDER, UE) for the funding through NanoSmart project (RYC-2016-21042) and for her postdoctoral contract within the Juan de la Cierva Program (JdC-2017), respectively.
Funding Information:
The authors acknowledge the CERIC–ERIC Consortium for the access to experimental facilities and financial support under Proposal CERIC-ERIC-20175403, and Dr. Heinz Amenitsch of Austrian SAXS beamline of the ELETTRA Synchrotron for the scientific and technical support provided during data collection experiments. J.M.D.-L and G.B.R.-R. acknowledge the Ministry of Science, Innovation and Universities of Spain (MCIU/AEI/FEDER, UE) for the funding through NanoSmart project (RYC-2016-21042) and for her postdoctoral contract within the Juan de la Cierva Program (JdC-2017), respectively.
Publisher Copyright:
© 2020 American Chemical Society.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/5/6
Y1 - 2020/5/6
N2 - Citrate and fluoride ions are two constituents of dental enamel hydroxyapatite (HA) nanocrystals. Their individual effect on HA crystallization has already been studied, and it was proven that both citrate and fluoride ions regulate HA crystal growth. However, the combined effect of citrate and fluoride ions on HA nanocrystals has never been reported so far. In this work, we have prepared citrate-fluoride-HA (citrate-FHA) nanoparticles in mild conditions, and we have studied the evolution of morphology and composition upon maturation. We have proven that even in the presence of citrate, fluoride ions are incorporated in the apatitic structure (replacing hydroxyl ions) and accelerate the crystallization process. Interestingly, citrate-FHA nanoparticles exhibit a flattened hexagonal rod-like morphology in contrast to the needle-like platelet morphology of citrate-HA. The density of citrate ions bound on the citrate-FHA surface is higher than that on citrate-HA. Moreover, the relative amount of unidentate citrate-Ca2+ adducts versus the ionic-like ones is higher for citrate-FHA than for citrate-HA. Our results provide a deeper understanding of the combined effect of citrate and fluoride ions on HA nanocrystals that can be used for the design of advanced biomaterials with tailored features, for a better comprehension of the enamel biomineralization process, and for the synthesis of enamel-like nanocrystals.
AB - Citrate and fluoride ions are two constituents of dental enamel hydroxyapatite (HA) nanocrystals. Their individual effect on HA crystallization has already been studied, and it was proven that both citrate and fluoride ions regulate HA crystal growth. However, the combined effect of citrate and fluoride ions on HA nanocrystals has never been reported so far. In this work, we have prepared citrate-fluoride-HA (citrate-FHA) nanoparticles in mild conditions, and we have studied the evolution of morphology and composition upon maturation. We have proven that even in the presence of citrate, fluoride ions are incorporated in the apatitic structure (replacing hydroxyl ions) and accelerate the crystallization process. Interestingly, citrate-FHA nanoparticles exhibit a flattened hexagonal rod-like morphology in contrast to the needle-like platelet morphology of citrate-HA. The density of citrate ions bound on the citrate-FHA surface is higher than that on citrate-HA. Moreover, the relative amount of unidentate citrate-Ca2+ adducts versus the ionic-like ones is higher for citrate-FHA than for citrate-HA. Our results provide a deeper understanding of the combined effect of citrate and fluoride ions on HA nanocrystals that can be used for the design of advanced biomaterials with tailored features, for a better comprehension of the enamel biomineralization process, and for the synthesis of enamel-like nanocrystals.
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U2 - 10.1021/acs.cgd.0c00038
DO - 10.1021/acs.cgd.0c00038
M3 - Article
AN - SCOPUS:85085149101
SN - 1528-7483
VL - 20
SP - 3163
EP - 3172
JO - Crystal Growth and Design
JF - Crystal Growth and Design
IS - 5
ER -