TY - JOUR
T1 - Comparative evaluation of torasemide and furosemide on rats with streptozotocin-induced diabetic nephropathy
AU - Arumugam, Somasundaram
AU - Sreedhar, Remya
AU - Miyashita, Shizuka
AU - Karuppagounder, Vengadeshprabhu
AU - Thandavarayan, Rajarajan A.
AU - Giridharan, Vijayasree V.
AU - Pitchaimani, Vigneshwaran
AU - Afrin, Rejina
AU - Harima, Meilei
AU - Suzuki, Kenji
AU - Watanabe, Kenichi
N1 - Funding Information:
This research was supported by a Ministry of Education, Culture, Sports, Science, and Technology , Japan, and by a grant from the Promotion and Mutual Aid Corporation for Private Schools , Japan ( 23602012 and 26460239 respectively).
PY - 2014/8
Y1 - 2014/8
N2 - Nephropathy is one of the complications of diabetes mellitus in human and experimental animals. There are various pathological renal remodeling processes leading to diabetic nephropathy because of the chronic hyperglycemia during diabetes mellitus. Various reports suggest the involvement of oxidative stress, inflammation and fibrosis during this progression. As antihypertensive drugs are reported to provide renoprotection in various animal models and clinical studies, we have carried out this study to identify the effect of torasemide, a loop diuretic, against streptozotocin-induced diabetic nephropathy and compare with furosemide. Here we have performed the measurement of blood and urine parameters and renal protein expression levels for measuring the disease severity in streptozotocin-induced diabetic rats treated torasemide or furosemide and compared with the vehicle treated rats. Furosemide treatment significantly increased the urinary protein excretion when compared with the normal rats. Torasemide treatment has reduced the expression of mineralocorticoid receptor and oxidative stress marker p67phox levels with improved mRNA levels of heme oxygenase-1 in the kidneys. In addition, torasemide treated diabetic rats showed significantly reduced expression of renal fibrosis related proteins when compared with the vehicle treated diabetic rats. Although furosemide treatment has produced improvement, its effects are comparably less than that of torasemide. Thus with the present results, we can suggest that torasemide treatment can improve the diabetic kidney disease in this rat model and which is superior to furosemide against renal fibrotic remodeling.
AB - Nephropathy is one of the complications of diabetes mellitus in human and experimental animals. There are various pathological renal remodeling processes leading to diabetic nephropathy because of the chronic hyperglycemia during diabetes mellitus. Various reports suggest the involvement of oxidative stress, inflammation and fibrosis during this progression. As antihypertensive drugs are reported to provide renoprotection in various animal models and clinical studies, we have carried out this study to identify the effect of torasemide, a loop diuretic, against streptozotocin-induced diabetic nephropathy and compare with furosemide. Here we have performed the measurement of blood and urine parameters and renal protein expression levels for measuring the disease severity in streptozotocin-induced diabetic rats treated torasemide or furosemide and compared with the vehicle treated rats. Furosemide treatment significantly increased the urinary protein excretion when compared with the normal rats. Torasemide treatment has reduced the expression of mineralocorticoid receptor and oxidative stress marker p67phox levels with improved mRNA levels of heme oxygenase-1 in the kidneys. In addition, torasemide treated diabetic rats showed significantly reduced expression of renal fibrosis related proteins when compared with the vehicle treated diabetic rats. Although furosemide treatment has produced improvement, its effects are comparably less than that of torasemide. Thus with the present results, we can suggest that torasemide treatment can improve the diabetic kidney disease in this rat model and which is superior to furosemide against renal fibrotic remodeling.
KW - Diabetic nephropathy
KW - Fibrosis
KW - Furosemide
KW - Inflammation
KW - Loop diuretic
KW - Torasemide
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U2 - 10.1016/j.yexmp.2014.06.007
DO - 10.1016/j.yexmp.2014.06.007
M3 - Article
C2 - 24960275
AN - SCOPUS:84903722350
SN - 0014-4800
VL - 97
SP - 137
EP - 143
JO - Experimental and Molecular Pathology
JF - Experimental and Molecular Pathology
IS - 1
ER -