TY - JOUR
T1 - Comparison Between Cancellous Trabecular and Cortical Specimens from Human Lumbar Spine Samples as an Alternative Source of Mesenchymal Stromal Cells
AU - Lenna, Stefania
AU - Brozovich, Ava
AU - Hirase, Takashi
AU - Paradiso, Francesca
AU - Weiner, Bradley K.
AU - Taraballi, Francesca
N1 - Funding Information:
Research reported in this publication was supported by internal seeding grant from the Orthopedic and Sports Medicine Department (OPPI) at Houston Methodist Hospital.
Publisher Copyright:
© Copyright 2022, Mary Ann Liebert, Inc., publishers 2022.
PY - 2022/11
Y1 - 2022/11
N2 - Due to their immunosuppressive potential and ability to differentiate into multiple musculoskeletal cell lineages, mesenchymal stromal cells (MSCs) became popular in clinical trials for the treatment of musculoskeletal disorders. The aim of this study was to isolate and characterize native populations of MSCs from human cortical and cancellous bone from the posterior elements of the lumbar spine and determine what source of MSCs yields better quality and quantity of cells to be potentially used for spinal fusion repair. We were able to show that MSCs from trabecular and cortical spine had the typical MSC morphology and expression markers; the ability to differentiate in adipocyte, chondrocyte, or osteoblast but they did not have a consistent pattern in the expression of the specific differentiation lineage genes. Moreover, MSCs from both sites demonstrated an immune suppression profile suggesting that these cells may have a more promising success in applications related to immunomodulation more than exploring their ability to drive osteogenesis to prevent nonunion in spine fusion procedures.
AB - Due to their immunosuppressive potential and ability to differentiate into multiple musculoskeletal cell lineages, mesenchymal stromal cells (MSCs) became popular in clinical trials for the treatment of musculoskeletal disorders. The aim of this study was to isolate and characterize native populations of MSCs from human cortical and cancellous bone from the posterior elements of the lumbar spine and determine what source of MSCs yields better quality and quantity of cells to be potentially used for spinal fusion repair. We were able to show that MSCs from trabecular and cortical spine had the typical MSC morphology and expression markers; the ability to differentiate in adipocyte, chondrocyte, or osteoblast but they did not have a consistent pattern in the expression of the specific differentiation lineage genes. Moreover, MSCs from both sites demonstrated an immune suppression profile suggesting that these cells may have a more promising success in applications related to immunomodulation more than exploring their ability to drive osteogenesis to prevent nonunion in spine fusion procedures.
KW - human lumbar spine samples
KW - immunosuppressive potential
KW - mesenchymal stromal cells
KW - multilineage differentiation ability
KW - spine fusion
UR - http://www.scopus.com/inward/record.url?scp=85141892699&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85141892699&partnerID=8YFLogxK
U2 - 10.1089/scd.2022.0157
DO - 10.1089/scd.2022.0157
M3 - Article
C2 - 36039931
SN - 1547-3287
VL - 31
SP - 672
EP - 683
JO - Stem Cells and Development
JF - Stem Cells and Development
IS - 21-22
ER -