Abstract
High-performance liquid chromatography (HPLC) was compared with gas chromatography-mass spectrometry (GC-MS) for quantitation of cocaine, benzoylecgonine, norcocaine, and cocaethylene in urine. Calibration standards were prepared in human urine, and bupivacaine was added as the internal standard for quantitation. After solid-phase extraction, the reconstituted samples were divided into aliquots for analysis by HPLC and GC-MS. The analytical performance of the two methods were compared with regard to sensitivity, precision, and dynamic range. Results of GC-MS and HPLC analyses of nine urine specimens previously confirmed as positive for benzoylecgonine were compared. Analytical results by HPLC were comparable to GC-MS. Therefore, for many laboratories, HPLC is a useful alternative to GC- MS for measuring cocaine and metabolites in urine.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 305-308 |
| Number of pages | 4 |
| Journal | Journal of Analytical Toxicology |
| Volume | 20 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jan 1 1996 |
ASJC Scopus subject areas
- Analytical Chemistry
- Environmental Chemistry
- Toxicology
- Health, Toxicology and Mutagenesis
- Chemical Health and Safety