Complement C3c and related protein biomarkers in amyotrophic lateral sclerosis and Parkinson's disease

Ira L. Goldknopf; Essam A. Sheta; Jennifer Bryson; Brian Folsom; Chris Wilson; Jeff Duty; Albert A. Yen; Stanley H. Appel

doi:10.1016/j.bbrc.2006.02.051

Complement C3c and related protein biomarkers in amyotrophic lateral sclerosis and Parkinson's disease

Ira L. Goldknopf, Essam A. Sheta, Jennifer Bryson, Brian Folsom, Chris Wilson, Jeff Duty, Albert A. Yen, Stanley H. Appel

Research output: Contribution to journal › Article › peer-review

109 Scopus citations

Abstract

We have used quantitative 2D gel electrophoresis to analyze serum proteins from 422 patients with neurodegenerative diseases and normal individuals in an unbiased approach to identify biomarkers. Differences in abnormal serum levels were found between amyotrophic lateral sclerosis (ALS), Parkinson's disease (PD), and related disorders for 34 protein biomarker spots, nine of which were related to the complement system. Of these nine, four spots originated from the Complement C3b-α-chain (C3c₁, C3c_2a, C3c _2b, and C3dg). The C3c spots (C3c₁, C3c_2a, and C3c_2b) had the same amino acid sequence and glycosylation, though only C3c₁ was phosphorylated. In addition, Complement Factors H, Bb, and Pre-Serum amyloid protein displayed different serum concentrations in ALS, PD, and normal sera, whereas Complement C4b γ-chain and Complement Factor I did not. The differential expression of the complement proteins provides potentially useful biomarkers as well as evidence for the involvement of inflammatory processes in the pathogenesis of ALS and PD.

Original language	English (US)
Pages (from-to)	1034-1039
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	342
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2006.02.051
State	Published - Apr 21 2006

Keywords

Amytrophic lateral sclerosis
Complement
Immune
Inflammation
LC-MS/MS
Neurodegenerative
Parkinson's
Phosphorylation
Proteomic
Two-dimensional electrophoresis

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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title = "Complement C3c and related protein biomarkers in amyotrophic lateral sclerosis and Parkinson's disease",

abstract = "We have used quantitative 2D gel electrophoresis to analyze serum proteins from 422 patients with neurodegenerative diseases and normal individuals in an unbiased approach to identify biomarkers. Differences in abnormal serum levels were found between amyotrophic lateral sclerosis (ALS), Parkinson's disease (PD), and related disorders for 34 protein biomarker spots, nine of which were related to the complement system. Of these nine, four spots originated from the Complement C3b-α-chain (C3c1, C3c2a, C3c 2b, and C3dg). The C3c spots (C3c1, C3c2a, and C3c2b) had the same amino acid sequence and glycosylation, though only C3c1 was phosphorylated. In addition, Complement Factors H, Bb, and Pre-Serum amyloid protein displayed different serum concentrations in ALS, PD, and normal sera, whereas Complement C4b γ-chain and Complement Factor I did not. The differential expression of the complement proteins provides potentially useful biomarkers as well as evidence for the involvement of inflammatory processes in the pathogenesis of ALS and PD.",

keywords = "Amytrophic lateral sclerosis, Complement, Immune, Inflammation, LC-MS/MS, Neurodegenerative, Parkinson's, Phosphorylation, Proteomic, Two-dimensional electrophoresis",

author = "Goldknopf, {Ira L.} and Sheta, {Essam A.} and Jennifer Bryson and Brian Folsom and Chris Wilson and Jeff Duty and Yen, {Albert A.} and Appel, {Stanley H.}",

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AU - Goldknopf, Ira L.

AU - Sheta, Essam A.

AU - Bryson, Jennifer

AU - Folsom, Brian

AU - Wilson, Chris

AU - Duty, Jeff

AU - Yen, Albert A.

AU - Appel, Stanley H.

PY - 2006/4/21

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N2 - We have used quantitative 2D gel electrophoresis to analyze serum proteins from 422 patients with neurodegenerative diseases and normal individuals in an unbiased approach to identify biomarkers. Differences in abnormal serum levels were found between amyotrophic lateral sclerosis (ALS), Parkinson's disease (PD), and related disorders for 34 protein biomarker spots, nine of which were related to the complement system. Of these nine, four spots originated from the Complement C3b-α-chain (C3c1, C3c2a, C3c 2b, and C3dg). The C3c spots (C3c1, C3c2a, and C3c2b) had the same amino acid sequence and glycosylation, though only C3c1 was phosphorylated. In addition, Complement Factors H, Bb, and Pre-Serum amyloid protein displayed different serum concentrations in ALS, PD, and normal sera, whereas Complement C4b γ-chain and Complement Factor I did not. The differential expression of the complement proteins provides potentially useful biomarkers as well as evidence for the involvement of inflammatory processes in the pathogenesis of ALS and PD.

AB - We have used quantitative 2D gel electrophoresis to analyze serum proteins from 422 patients with neurodegenerative diseases and normal individuals in an unbiased approach to identify biomarkers. Differences in abnormal serum levels were found between amyotrophic lateral sclerosis (ALS), Parkinson's disease (PD), and related disorders for 34 protein biomarker spots, nine of which were related to the complement system. Of these nine, four spots originated from the Complement C3b-α-chain (C3c1, C3c2a, C3c 2b, and C3dg). The C3c spots (C3c1, C3c2a, and C3c2b) had the same amino acid sequence and glycosylation, though only C3c1 was phosphorylated. In addition, Complement Factors H, Bb, and Pre-Serum amyloid protein displayed different serum concentrations in ALS, PD, and normal sera, whereas Complement C4b γ-chain and Complement Factor I did not. The differential expression of the complement proteins provides potentially useful biomarkers as well as evidence for the involvement of inflammatory processes in the pathogenesis of ALS and PD.

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KW - Immune

KW - Inflammation

KW - LC-MS/MS

KW - Neurodegenerative

KW - Parkinson's

KW - Phosphorylation

KW - Proteomic

KW - Two-dimensional electrophoresis

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Complement C3c and related protein biomarkers in amyotrophic lateral sclerosis and Parkinson's disease

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Keywords

ASJC Scopus subject areas

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