TY - JOUR
T1 - Complex nanostructures based on oligonucleotide optical switches and nanoparticles for intracellular mRNA sensing and silencing
AU - Adinolfi, B.
AU - Carpi, S.
AU - Giannetti, A.
AU - Nieri, P.
AU - Pellegrino, M.
AU - Sotgiu, G.
AU - Tombelli, S.
AU - Trono, C.
AU - Varchi, G.
AU - Baldini, F.
N1 - Funding Information:
This work was supported by the regional project NANOCELL (PAR FAS REGIONE TOSCANA Linea 1.1.a.3) and by the national flagship project NANOMAX.
Publisher Copyright:
© 2014 The Authors. Published by Elsevier Ltd.
PY - 2014
Y1 - 2014
N2 - The design, implementation and characterization of complex nanostructures for intracellular mRNA monitoring constituted by a molecular beacon (MB), covalently immobilized onto polymethylmethacrylate (PMMA) fluorescent nanoparticles (NPs) are described. The MB molecule, adsorbed onto the nanoparticle for intracellular delivery, is capable to turn on the fluorescence emission of its fluorescent label, upon the interaction with a well-defined target mRNA. The mRNA specific for survivin has been chosen as target. A MB for survivin mRNA was characterized in vitro to verify its functionality. The functionality and specificity of the MB were also examined in living cells by transfection with a classical lipid agent, Lipofectamine. In addition, bear and MB-coated PMMA NPs were tested with adenocarcinomic human alveolar basal epithelial cells (A549) in terms of cell vitality and internalization.
AB - The design, implementation and characterization of complex nanostructures for intracellular mRNA monitoring constituted by a molecular beacon (MB), covalently immobilized onto polymethylmethacrylate (PMMA) fluorescent nanoparticles (NPs) are described. The MB molecule, adsorbed onto the nanoparticle for intracellular delivery, is capable to turn on the fluorescence emission of its fluorescent label, upon the interaction with a well-defined target mRNA. The mRNA specific for survivin has been chosen as target. A MB for survivin mRNA was characterized in vitro to verify its functionality. The functionality and specificity of the MB were also examined in living cells by transfection with a classical lipid agent, Lipofectamine. In addition, bear and MB-coated PMMA NPs were tested with adenocarcinomic human alveolar basal epithelial cells (A549) in terms of cell vitality and internalization.
KW - MRNA
KW - Molecular beacon
KW - PMMA nanoparticle
KW - Survivin
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U2 - 10.1016/j.proeng.2014.11.645
DO - 10.1016/j.proeng.2014.11.645
M3 - Conference article
AN - SCOPUS:84923319787
SN - 1877-7058
VL - 87
SP - 751
EP - 754
JO - Procedia Engineering
JF - Procedia Engineering
T2 - 28th European Conference on Solid-State Transducers, EUROSENSORS 2014
Y2 - 7 September 2014 through 10 September 2014
ER -