TY - JOUR
T1 - Contribution of the enterococcal surface protein Esp to pathogenesis of Enterococcus faecium endocarditis
AU - Heikens, Esther
AU - Singh, Kavindra V.
AU - Jacques-Palaz, Karen D.
AU - van Luit-Asbroek, Miranda
AU - Oostdijk, Evelien A.N.
AU - Bonten, Marc J.M.
AU - Murray, Barbara E.
AU - Willems, Rob J.L.
N1 - Funding Information:
EH and RW were supported by the European Union Sixth Framework Programme “Approaches to Control multi-resistant Enterococci (ACE): Studies on molecular ecology, horizontal gene transfer, fitness and prevention” under contract LSHE-CT-2007-037410 and ZonMW “Vaccine-development to combat the emergence of vancomycin-resistant Enterococcus faecium ” project number 0.6100.0008 . BEM and KVS were supported in part by NIH grant R01 AI067861 to BEM from the Division of Microbiology and Infectious Diseases, NIAID.
PY - 2011/12
Y1 - 2011/12
N2 - The enterococcal surface protein Esp, specifically linked to nosocomial Enterococcus faecium, is involved in biofilm formation. To assess the role of Esp in endocarditis, a biofilm-associated infection, an Esp-expressing E. faecium strain (E1162) or its Esp-deficient mutant (E1162Δ. esp) were inoculated through a catheter into the left ventricle of rats. After 24 h, less E1162Δ. esp than E1162 were recovered from heart valve vegetations. In addition, anti-Esp antibodies were detected in Esp-positive E. faecium bacteremia and endocarditis patient sera. In conclusion, Esp contributes to colonization of E. faecium at the heart valves. Furthermore, systemic infection elicits an Esp-specific antibody response in humans.
AB - The enterococcal surface protein Esp, specifically linked to nosocomial Enterococcus faecium, is involved in biofilm formation. To assess the role of Esp in endocarditis, a biofilm-associated infection, an Esp-expressing E. faecium strain (E1162) or its Esp-deficient mutant (E1162Δ. esp) were inoculated through a catheter into the left ventricle of rats. After 24 h, less E1162Δ. esp than E1162 were recovered from heart valve vegetations. In addition, anti-Esp antibodies were detected in Esp-positive E. faecium bacteremia and endocarditis patient sera. In conclusion, Esp contributes to colonization of E. faecium at the heart valves. Furthermore, systemic infection elicits an Esp-specific antibody response in humans.
KW - Endocarditis
KW - Enterococcal surface protein Esp
KW - Enterococcus faecium
KW - Immunity
KW - Pathogenesis
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U2 - 10.1016/j.micinf.2011.08.006
DO - 10.1016/j.micinf.2011.08.006
M3 - Article
C2 - 21911077
AN - SCOPUS:81155122933
SN - 1286-4579
VL - 13
SP - 1185
EP - 1190
JO - Microbes and Infection
JF - Microbes and Infection
IS - 14-15
ER -