TY - JOUR
T1 - Cutting edge
T2 - CTLA-4Ig inhibits memory B cell responses and promotes allograft survival in sensitized recipients
AU - Chen, Jianjun
AU - Wang, Qiang
AU - Yin, Dengping
AU - Vu, Vinh
AU - Sciammas, Roger
AU - Chong, Anita S.
N1 - Publisher Copyright:
Copyright © 2015 by The American Association of Immunologists, Inc.
PY - 2015/11
Y1 - 2015/11
N2 - Sensitized recipients with pretransplant donor-specific Abs are at higher risk for Ab-mediated rejection than nonsensitized recipients, yet little is known about the properties of memory B cells that are central to the recall alloantibody responses. Using cell enrichment and MHC class I tetramers, C57BL/6 mice sensitized with BALB/c splenocytes were shown to harbor H-2Kd-specific IgG+ memory B cells with a post-germinal center phenotype (CD73+CD273+CD38hiCD138-GL7-). These memory B cells adoptively transferred into naive mice without memory T cells recapitulated class-switched recall alloantibody responses. During recall, memory H-2Kd-specific B cells preferentially differentiated into Ab-secreting cells, whereas in the primary response, H-2Kd-specific B cells differentiated into germinal center cells. Finally, our studies revealed that, despite fundamental differences in alloreactive B cell fates in sensitized versus naive recipients, CTLA-4Ig was unexpectedly effective at constraining B cell responses and heart allograft rejection in sensitized recipients.
AB - Sensitized recipients with pretransplant donor-specific Abs are at higher risk for Ab-mediated rejection than nonsensitized recipients, yet little is known about the properties of memory B cells that are central to the recall alloantibody responses. Using cell enrichment and MHC class I tetramers, C57BL/6 mice sensitized with BALB/c splenocytes were shown to harbor H-2Kd-specific IgG+ memory B cells with a post-germinal center phenotype (CD73+CD273+CD38hiCD138-GL7-). These memory B cells adoptively transferred into naive mice without memory T cells recapitulated class-switched recall alloantibody responses. During recall, memory H-2Kd-specific B cells preferentially differentiated into Ab-secreting cells, whereas in the primary response, H-2Kd-specific B cells differentiated into germinal center cells. Finally, our studies revealed that, despite fundamental differences in alloreactive B cell fates in sensitized versus naive recipients, CTLA-4Ig was unexpectedly effective at constraining B cell responses and heart allograft rejection in sensitized recipients.
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U2 - 10.4049/jimmunol.1500940
DO - 10.4049/jimmunol.1500940
M3 - Article
AN - SCOPUS:84945137648
SN - 0022-1767
VL - 195
SP - 4069
EP - 4073
JO - Journal of Immunology
JF - Journal of Immunology
IS - 9
ER -